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MACS/StraightFrom LRSC CD4 MicroBead Kit, human/130-117-028/188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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MACS/StraightFrom LRSC CD4 MicroBead Kit, human/130-117-028/

Capacity:for1LRSC
Components:
  • 2mLStraightFromLRSCCD4MicroBeads
  • 12×LSColumns
  • WholeBloodColumnElutionBuffer

Overview

TheStraightFrom™MicroBeadKitwasdevelopedfortherapidpositiveselectionofCD4+TcellsdirectlyfromoneLRSCusingtheMultiMACS™Cell24SeparatorPlusforsemi-automatedseparationoraMACS®Separatorformanualseparation.Nosamplepreparationisrequired,includingdensitygrADIentcentrifugation,erythrocytelysis,washsteps,orcellcount.LSColumnsandWholeBloodColumnElutionBufferareincludedinthekittoallowdirectprocessingofanentireLRSC.

Details

Backgroundinformation

TheCD4antigenisexpressedonThelpercellsandatalowerlevelonmonocytesanddendriticcells.TheCD4antigenisanaccessorymoleculeintherecognitionofMHCclassII/peptidecomplexesbytheTCRheterodimeronCD4+Thelpercells.

Detailedseparationprocedure

OneentireLRSCsampleislabeledwithStraightFrom™LRSCCD4MicroBeadsandloadedontoLSColumns,whichareplacedinthemagneticfieldofaMultiMACS™Cell24SeparatorPlusoraMACS®Separator.Afterremovingthecolumnfromthemagneticfield,themagneticallyretainedCD4+Tcellscanbeelutedasthepositivelyselectedcellfraction.

Downstreamapplications

ThepurifiedCD4+Tcellsarewellsuitedforfurtherflowcytometric,functional,ormolecularanalysis.
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