Expressionsystems/ESF 921 Insect Cell Culture Medium, Protein Free/96-001-10/1 Ea

ESF 921 Insect Cell Culture Medium, Protein Free10L Media Transfer Bag

ESF 921™ Insect Cell Culture Medium is a complete serum-free, protein-free medium developed for robust cell growth, protein production and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni (High Five™), and Drosophila S2. ESF 921 contains L-Glutamine and Kolliphor® P188 (Pluronic F68).

Description:

Widely recognized as the industry standard for BEVS applications, Expression Systems insect cell culture media are designed for maximum virus and protein expression. This serum-free, protein-free insect cell culture medium provides unmatched cell growth and expression for a wide range of insect cells including Sf9, Sf21, Tni (High Five^TM) and Drosophila S2.

• Sf9 cell densities of 15 x 10⁶ cells/ml or greater
• Produces high titer baculovirus vector yields of 5E8 to 3E9 infectious units/ml
• Available in 1L bottles, bulk packaging and single-use bags that eliminate the need to transfer media
• Complete, ready-to-use, contains L-glutamine
• Ideal for scale-up manufacturing
• Works with a wide variety of baculovirus and host cell platforms
• In stock for immediate shipment
• Adapted cells available

Superior Growth and Reproducibility

Expression Systems’ ESF 921^TM Insect Cell Culture Medium is designed for maximum expression for applications such as; gene therapy drug substance, virus-like particles, virus vectors and proteins of interest. ESF 921^TM is widely recognized as the industry standard for the baculovirus and insect host cell system. The line graph presents reproducible growth curves for Sf9 cell cultures in nine different lots of ESF 921^TM medium manufactured over a period of 12 months.

Sf9 cells from a mid-log phase culture were seeded into *9 different lots of ESF 921^TM at a density of 0.75×10,⁶/ml. Cultures were sampled daily to determine viable cell count.

Specification:

Media type	Ready-to-use serum-free liquid, 1X
Platform compatibility	Functions with BestBac™, Bacmid, Bac-to-Bac®, BacMam™, MultiBac™ and other BEVS platforms
Cell lines	Spodoptera frugiperda (Sf9, Sf21), Trichoplusia ni (Tni and High Five™) and Drosophila melanogaster (S2)
Use-by date	One year from date of manufacture

RecommendedStorage Condition

2–8º C