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Tribioscience/Fast Glucose Determination Assay (Colorimetric/Fluorometric)/200 Assays/TBS2087-200

Glucose is the most important carbohydrate in biology. It is a primary source of energy for the body’s cells transported through the bloodstream. As such, glucose levels need to be highly regulated in the human body. Failure to regulate blood glucose within the normal range leads to conditions of persistently high or low blood sugar. Diabetes mellitus is the most prominent disease related to improper blood sugar regulation. The determination of glucose levels in the blood is critical in the control of diabetes. Tribio’s™ Fast Glucose Determination Assay (Colorimetric/Fluorometric) provides a rapid, simple, reproducible, and sensitive approach for measuring glucose in plasma, serum, urine, and other bio-samples. The glucose assay uses the glucose oxidase-peroxide reaction to measure glucose concentrations. The color intensity of the reaction product at 570nm or fluorescence intensity at λ em/ex=585/530 nm is directly proportional to the glucose concentration in the sample.

Applications:

Direct Assays: Glucose in serum, plasma, urine, and other bio-samples.

Key Features:

Flexible: Suitable for colorimetric and fluorometric methods. Accurate: Use 50μL samples. Detection ranges from 0.4-200 μM in a 96-well plate for colorimetric assay and 1-50 μM for fluorometric assay. Simple and High-Throughput: One-step procedure: load, incubate, read. The kit can be used for a robust method. Time Saving: Takes less than 30 minutes

Kit Contents:

(for 100 Assays)

ComponentTBS2087-200TBS2087-600
5x Assay Buffer1x 12mL3x 12mL
Red Probe1x 0.25mL1x 0.8mL
Glucose Standard Stock (2mM)1x 100μL1x 300μL
Enzymes1x 2mL3x 2mL
Storage and Handling:

Store kit at -20℃. Shelf life of 6 months. Protect from light.

Example Colorimetric Standard Curve: Image of Colorimetric Standard Curve

Example Fluorometric Standard Curve: Image of Fluorometric Standard Curve

Additional information
SIZE

200 Assays, 600 Assays

TBS2087_Fast_Glucose_Colorimetric/Fluorometric


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