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Normal human serum depleted of complement C8 protein by immunoaffinity chromatography. The product is tested for the absence of C8 by functional assays for classical pathway activity and alternative pathway activity and for C8 protein by double immunodiffusion. C8-Dpl is certified to possess functional classical and alternative pathways for complement activation up to the step of C8 binding to C5b67 (Law, S.K.A. and Reid, K.B.M. (1995); Morgan, B.P. ed. (2000); Dodds, A.W. and Sim, R.B. editors (1997)).Functional complement systems can be reconstituted by addition of purified C8 protein (55 µg/mL) indicating that all other complement components necessary for complement activation are present.
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