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ImmunoChem/Acetyl Lysine Antibody, Agarose -2mg/2 mg/ICP0388-2MG

Acetyl Lysine Antibody, Agarose -2mg

Catalog # Pack Size Price(USD)
ICP0388-2MG 2 mg $550.00

Quantity:

Product Description

The acetylated lysine antibody is immobilized to agarose beads via amide linkages. The product can be utilized as an affinity matrix for rapid isolation and purification of proteins/peptides with acetyl lysine residues.

The acetylated peptides eluted from acetyl-lysine-specific affinity chromatography (ICP0388).
Approximately 5 mg of trypsinated crude protein from TSA treated MMRU cells wereloaded.
The maximum binding of acetylated BSA with ICP0388-2MG.
-50 µl of ICP0388-2MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min.
- After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl
-5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE and blotted wih monoclonal Mouse anti-Acetylated Lysine (ICP0390)
Formulation

0.5 mL of agarose beads suspended in 1 mL of slurry

Antibody Immobilized

4 mg/ml antibody is covalently linked through amide bonds with NHS activated-SMCC, which further conjugates to thiolated agarose beads via thiol ether bonds.

Specificity

The antibody selectively captures proteins/peptides wtih acetylated lysine residues (N-epsilon). No cross reactions to methylated proteins/peptides.

BindingCapacity

Approximately 0.2 mg of acetylated histone / mL slurry.
Applications

Rapid isolation and purification of proteins/peptides with acetylated lysine residues from cell lysate or protease-digested mixtures.

Scientific Description

Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the acetylated species for further MS/MS identification.

Storage & Stability

Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Shake thoroughly before use.Expiration date is one year from date of shipping if properly stored.

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