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Clickchemistrytools/5-Ethynyl Uridine (5-EU)/1261-25/25 mg

5-Ethynyluridine(5-EU)isametaboliclabelingreagentusedtodetectglobalRNAtranscriptiontemporallyandspatiallyinbothin-vitroandin-vivowiththemodifiednucleotideusingtwo-steptechnique.

Instepone,thealkyne-containinguridineanalog5-EUisfedtocellsoranimalsandactivelyincorporatedintoRNA.Insteptwotheincorporated5-EUisdetectedviachemoselectiveligationor“click”reactionbetweenanazideandanalkynewherethemodifiedbiomoleculesaredetectedwithacorrespondingazide-containingdyeorhapten.Cellscanbeanalyzedbyfluorescencemicroscopy,flowcytometryorhigh-contentimagingandanalysis(HCS).


Molecularweight268.22
ChemicalcompositionC11H12N2O6
CAS69075-42-9
SolubilityWater,DMSO,DMF
Purity>95%(HPLC)
AppearanceOffwhitetogreycrystalline
Storageconditions-20C
ShippingconditionsAmbienttemperature
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