Anti-CD80 Antibody (16-10A1) – APC
Product Benefits:
ichorbio’s Anti-CD80 Antibody (16-10A1) – APC is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Target:
CD80
Clone:
16-10A1
Isotype:
Armenian Hamster IgG
Other Names:
Activation B7-1 antigen, B7
Uniprot:
Q00609
Host:
Armenian Hamster
Species Reactivity:
Mouse, Rat
Specificity:
Anti-CD80 Antibody (16-10A1) – APC recognizes an epitope on Mouse CD80
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.
Background:
CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.
Immunogen:
Purified Recombinant Mouse CD80 (>98%)
Concentration:
0.1mg/ml
Formulation:
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage:
This APC antibody is stable when stored at 2-8°C. Do not freeze.
Applications:
Flow Cytometry
Application Notes:
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD80. A suggested method would be to stain cells expressing CD80 with <0.5 µg per 100 µl of whole blood (or per 1.0 x 10^6 cells in a 100 µl total staining volume). Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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