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Product Name：MxSafe Plus（Security nucleic acid dye）
Catalog Number: Mx017
HeLa cells were incubated at 37oC with 1X of SYBR Green I, MxSafe and MxSafe Plus,respectively. Images were taken
following incubation for5 min (panel A) and 30 min (panel B), respectively.SYBR Green I entered into cells rapidly as ev
-ident from the bright green nuclear staining.However, MxSafe and MxSafe Plus were unable to cross cell membranes
as shown by the lack of any fluorescence staining.
MxSafe Plusis an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace
thehighly toxicethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.MxSafePlus
is farmore sensitivethan EB without requiring a destaining step.MxSafe Plusand EB have virtually the same spectra, so you
can directlyreplace EBwithMxSafe Pluswithout changing your existing imaging system.
MxSafe Plusis a very stable dye. Store 10,000X solution and dilute solutions of MxSafe Plus at room temperature,
protectedfrom light. Dye precipitation may occur at lower temperatures, resulting in lower signalorthe
appearance ofprecipitate on the surface of the gel. If this occurs, heat the solution to 45-50oC for twominutes
and vortex.MxSafe Plusis stable for at least one year from the date it is received.
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