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Product Name：MxSafe（Security nucleic acid dye）
Catalog Number: Mx007
HeLa cells wereincubated at 37 oC with 1X of SYBR Green I, MxSafe and MxSafe Plus,respectively.Images were taken
following incubation for5 min (panel A) and 30 min (panelB), respectively.SYBR Green I entered into cells rapidly as ev
-ident from thebright green nuclear staining.However, MxSafe and MxSafe Plus were unable tocross cell membranes
as shown by the lack of any fluorescence staining.
MxSafe is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace
thehighly toxicethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. MxSafe
is farmore sensitivethan EB without requiring a destaining step. MxSafeand EB have virtually the same spectra, so you
can directlyreplace EBwithMxSafewithout changing your existing imaging system.
MxSafe is a very stable dye. Store 10,000X solution and dilute solutions ofMxSafe at room temperature, protected
from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of
precipitate onthe surface of the gel. If this occurs, heat the solution to 45-50oC for two minutesand vortex.
MxSafeis stable for at least one year from the date it is received.
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