5-Aminoallyl-dUTP(AA-dUTP)canbeenzymaticallyincorporatedintoDNA.Theresultingamine-containingDNAcanbesubsequentlylabeledwithafluorescentdye,biotinorotherhaptensviaconventionalpeptidecouplingmethod(1,2).Thistwo-stepmethodforlabelingnucleicacidsisconsiderablymoreeconomicalthantheone-stepmethodusingaprelabeleddUTP.
AA-dUTPisalsoavailableinlyophilizedform(catalogno.40020-1).
1.BiolChemHoppeSeyler371,953(1990).
2.Biotechniques28,518(2000).
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g