Hooke labs/Control and naive tissue, cells, and serum/DBA/1-F/CZ-0010

Control and naive tissue, cells, and serum

For analysis of gene expression and protein production

Availability | Preparation | CIA |EAE |Naïve |Contents | Storage | SDS |Related products

Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).

These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.

• Analyze gene expression, protein presence, and biomarkers without a vivarium

Save time establishing & running disease models.

• All products can be customized to your requirements

Contactorfor pricing and details.

Availability and lead time (IMPORTANT)

Most frozen products are available from stock with no lead time, but contact us atorfor current inventory.

Fresh cell orders must be placed 3 weeks before shipment. Each cell product order is custom prepared.

Note: Brain tissue is also available for some mice (add $17/mouse).See our current inventory for availability.

CIA control tissue and serum

Snap frozen tissue from mice immunized with adjuvant only (control kit; no CIA induction), matched for time from CIA induction.

(See also CIA tissue.)

Frozen serum from mice immunized with adjuvant only (control kit).

(See also CIA serum.)

EAE control tissue and cells

Snap frozen tissue from mice immunized with adjuvant only (control kit; no EAE induction), matched for time from EAE induction.

(See also EAE tissue.)

Fresh cells from mice immunized with adjuvant only (control kit; no EAE induction). Termination at age 8 to 18 weeks.

Naïve tissue, cells, and serum

Snap frozen tissue from naïve mice (no immunization or disease induction).

Fresh cells from naïve mice (no immunization or disease induction). Termination at age 8 to 18 weeks.

Frozen serum from naïve mice (no immunization or disease induction).

Preparation

Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.

For products from immunized mice (does not apply to products from naïve mice), mice are then immunized with adjuvant(s) only as follows:

Mice are euthanized at the timepoints given above.

Snap frozen tissue products

If serum is included, blood is collected immediately after termination and serum is isolated.

All tissue is snap frozen in liquid nitrogen and stored at -80 °C until shipment.

Fresh cell products

Cell suspensions are prepared and red blood cells in spleen are lysed.

Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

Cells are shipped the same day for overnight delivery.

Frozen serum products

At termination blood is immediately collected, and serum is isolated.

Serum from multiple mice is pooled and stored at -80 °C until shipment.

Detailed contents - snap frozen tissue

Each product unit contains tissue and/or serum from a single mouse.

Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID, paw ID (left or right hind paw; if applicable), and tissue weight.

Typical tissue quantity:

• Spinal cord 80 to 100 mg
• Spleen 100 to 200 mg
• Half spleen 40 to 80 mg
• Hind paw 100 to 150 mg
• Serum 100 µL

Detailed contents - fresh cells

Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.

Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10^-5 M 2-mercaptoethanol).

Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.

(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)

The typical number of cells expected for naïve and immunized mice is as follows:

Detailed contents - frozen serum

Frozen serum, pooled from multiple mice, supplied in a 1.5 mL cryogenic tube on dry ice.

Storage & stability

Frozen tissue and serum - keep frozen until use. Storage at -80 °C is recommended.

Fresh cells - These ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight.

(We can supply frozen cells to international customers at an additional charge of $27/item. These ship on dry ice. Please specify "frozen cells" when ordering.)

Safety Data Sheets (SDS)

Frozen tissue and serum SDS (PDF)Fresh cell products SDS (PDF)

Related products

Tissue from mice with CIASerum from mice with CIATissue from mice with EAEControl and naïve tissue, cells, and serum