Hooke labs/Tissue and cells from mice with EAE/C57BL/6/EZ-1110

Tissue and cells from mice with EAE

For analysis of gene expression and protein production

Availability & Lead Time | Preparation | Contents | Storage | SDS (pdf) | Related products

Snap frozen mouse tissue from well-defined stages and severity of EAE, and fresh cells from EAE-immunized mice. Choose tissue samples from our inventory based on EAE severity, disease stage, or mouse strain. Tissue shipped frozen on dry ice, fresh cells shipped on gel packs.

• Analyze gene expression and protein presence in EAE tissue without a vivarium

Save time establishing & running EAE models; most tissue products are available from stock with no lead time.

• Choose individual mice from our inventory based on EAE severity

Full EAE score history provided for each sample.

• Use fresh cells for adoptive transfer or T cell function analysis.

Suspensions shipped cold overnight, ready for use.

• Tissue from control and naïve mice available for comparison

Control tissues are matched for immunization method and timing.

Availability and lead time (IMPORTANT)

Most tissue products are available from stock with no lead time, but contact us atorfor a complete listing of our current inventory in a spreadsheet, including EAE score history for each mouse.

Choose individual mice from our stock based on EAE severity, disease stage, or mouse strain.

Fresh cell orders must be placed 3 weeks before shipment. Each cell product order is custom prepared.

Note: Brain tissue is also available for some mice (add $17/mouse).See our current inventory for availability.

Tissue from mice with EAE - snap frozen

(See also control and naïve tissue.)

Cells from MOG or PLP-immunized mice - fresh

(See also control and naïve tissue.)

Preparation - frozen tissue

Female C57BL/6 or SJL mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

In C57BL/6 mice, EAE is induced according to the protocol EAE Induction by Active Immunization in C57BL/6 Mice.

In SJL mice, EAE is induced according to the protocol EAE Induction by Active Immunization in SJL Mice.

Mice are scored daily for EAE severity on the scale of 0-5 in 0.5 unit increments (see protocol), and weighed 3 times/week (Monday, Wednesday, Friday).

Mice are euthanized at the appropriate stage of disease, immediately perfused with PBS, then spleen & spinal cord are isolated and snap frozen in liquid nitrogen.

Tissues are stored at -80 °C until shipment.

Preparation - fresh cells

Female mice of age 10 to 14 weeks are acclimated to our facility for at least 7 days, then immunized with antigen subcutaneously in the lower back, close to the base of tail, as follows:

11 days later (10 days for SJL mice), mice are euthanizedand cell suspension(s) from spleen and/or inguinal lymph nodes are prepared.

Red blood cells in spleen are lysed.

Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

Cells are shipped the same day for overnight delivery.

Detailed contents - frozen tissue

Each product unit contains the entire spleen and spinal cord tissue from a single mouse.

Each organ is supplied in a separate 1.5 mL cryogenic tube.Tubes are marked with a mouse ID and tissue weight. (Typical spinal cords are 80 to 100 mg each, spleens are typically 100 to 200 mg.)

The daily EAE severity scores and weight history for the mouse are provided for each mouse in a spreadsheet, via email. (Normally customer chooses mice for ordering based on EAE score history.)

Detailed contents - fresh cells

Centrifuge tubes (15 or 50 mL) with cells in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10^-5 M 2-mercaptoethanol).

Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered,each type of cells will be pooled in one tube with one cell count.

(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)

Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.

Storage & stability

Frozen tissue - keep frozen until use. Storage at -80 °C is recommended.

Fresh cells ship cold on gel packs in insulated packaging. They should be used or frozen within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight.

(We can supply frozen cells to international customers at an additional charge of $27/item. These ship on dry ice. Please specify "frozen cells" when ordering.)

Safety Data Sheet (SDS)

File link (PDF)

Related products

Control and naïve tissue, cells, and serum Hooke Kits™ for EAE Induction in C57BL/6 Mice DS-0111 MOG_35-55 in TC Media, 100x DS-0121 [Ser¹⁴⁰]-PLP_139-151 in TC Media, 100x DS-0161 PLP_139-151 (native) in TC Media, 100x