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For analysis of gene expression, protein production and biomarkers
Snap frozen mouse paw, spleen, and serum from DBA/1 mice at well-defined stages and severity of CIA. Choose paws from our inventory based on CIA score history, disease stage, gender, or antigen used for CIA induction. Shipped frozen on dry ice.
Save time establishing & running CIA models; most tissue products are available from stock with no lead time.
Full CIA score history provided for each sample.
Control tissues are matched for immunization method and timing.
Most tissue products are available from stock with no lead time, but contact us atorfor a complete listing of our current inventory in a spreadsheet, including CIA score history for each mouse.
You can choose individual left or right hind paws from our stock based on CIA severity, disease stage, gender, or antigen used for CIA induction (chicken type II collagen or bovine type II collagen).
| Cat # | Strain-Gender | Disease stage | Tissue | Size | Price |
|---|---|---|---|---|---|
| CZ-0110 | DBA/1-F | 24-48 hours after CIA onset | Hind paw, spleen, serum | Half mouse | $199 |
| CZ-0111 | DBA/1-M | 24-48 hours after CIA onset | Hind paw, spleen, serum | Half mouse | $157 |
| CZ-0120 | DBA/1-F | 4-5 days after CIA onset | Hind paw, spleen, serum | Half mouse | $199 |
| CZ-0121 | DBA/1-M | 4-5 days after CIA onset | Hind paw, spleen, serum | Half mouse | $157 |
| CZ-0130 | DBA/1-F | 2-3 weeks after CIA onset | Hind paw, spleen, serum | Half mouse | $199 |
| CZ-0131 | DBA/1-M | 2-3 weeks after CIA onset | Hind paw, spleen, serum | Half mouse | $157 |
(See also control and naïve tissue.)
Male or female DBA/1 mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.
CIA is induced according to the protocol CIA Induction in DBA/1 Mice.
Each paw is scored daily for CIA severity on the scale of 0-4 (see protocol) until disease onset, and then every other day. Total score for each mouse is calculated as the sum of scores for all 4 paws (0-16).
Mice are euthanized at the appropriate stage of disease.Immediately, blood is collected for serum, hind paws and spleen are collected and snap frozen in liquid nitrogen.Serum is isolated and frozen at -80 °C.
Tissues are stored at -80 °C until shipment.
| Qty | Description |
|---|---|
| 1 | Hind paw, frozen in 1.5 mL cryogenic tube. Typical CIA paw weight 100-400 mg.Typical control or naïve paw weight 100-150 mg. |
| 1 | Half spleen, frozen in 1.5 mL cryogenic tube. Typical weight 40-80 mg. |
| 100 µL | Serum, frozen in 1.5 mL cryogenic tube. |
| 1 | Spreadsheet (via email) with CIA score history for all 4 paws. |
Each product unit contains tissue and serum from a single mouse.(Spleens and serum are divided and packaged with a single paw because paws of the same mouse can have different arthritis severity.)
Tubes are marked with a mouse ID, paw ID (left or right hind paw), and weight of paw or spleen tissue.
Normally customer orders tissue samples based on CIA score history.
Keep frozen until use. Storage at -80 °C is recommended.
File link (PDF)
Serum from mice with CIAControl and naïve tissue, cells, and serum
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g