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BioAssay Systems/EnzyChrom™ ADP Assay Kit/100 tests/E2ADP-100

EnzyChrom™ ADP Assay Kit

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ProtocolSDS

Application

For quantitative determination of ADP determination in cells and other biological samples.

Key Features

Safe. Non-radioactive assay.
Sensitive and accurate. As low as 0.1 μM ADP can be quantified.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

FL530/590nm

Samples

Cells and other biological samples

Species

Size

100 tests

Detection Limit

0.1 µM

Shelf Life

6 months

More Details

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal. BioAssay Systems' newly designed ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorimetric method (lexc/em = 530/590nm). The assay is simple, sensitive, stable, high-throughput adaptable and can detect as low as 0.1 µM ADP in biological samples.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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