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BioAssay Systems/EnzyChrom™ Acetate Assay Kit/100 tests/EOAC-100

EnzyChrom™ Acetate Assay Kit

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ProtocolSDS

Application

For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.

Key Features

Use as little as 10 μL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.

Method

OD570nm, or FL530/585nm

Samples

Serum, plasma, food, agriculture and environment

Species

Size

100 tests

Detection Limit

0.13 mM

Shelf Life

6 months

More Details

Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. BioAssay Systems assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Inagaki, T., & Matsuura, K. (2018). Extended mutualism between termites and gut microbes: nutritional symbionts contribute to nest hygiene. The Science of Nature, 105(9-10), 52. Assay: Acetate in termite gut content. Smith, W. B., Gibson, S., Newman, G. E., Hendon, K. S., Askelson, M., Zhao, J., & Thadhani, R. I. (2017). The dynamics of the metabolism of acetate and bicarbonate associated with use of hemodialysates in the ABChD trial: a phase IV, prospective, single center, single blind, randomized, cross-over, two week investigation. BMC Nephrology, 18(1), 273. Assay: Acetate in human blood. Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in microbe culture. Garrido D et al (2013). Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiol. 33(2):262-70. Assay: Acetate in microbe cell.To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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