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Pazopanib HCl (GW786034 HCl) | 购买VEGFR 抑制剂 - Selleck188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Pazopanib HCl (GW786034 HCl) | 购买VEGFR 抑制剂 - Selleck

定制您的化合物库 通过在我们的库存中挑选化合物来建立合适的化合物库,用于您的研究工作。 您可以选择: 特定化合物 96/384孔板位图 可选择\"干/固体”或\"DMSO溶液” 请通过info@selleck.cn与我们联系,定制你所需要的化合物库。 Pazopanib HCl (GW786034 HCl)是一种新型多靶点抑制剂,作用于VEGFR1,VEGFR2,VEGFR3,PDGFR,FGFR,c-Kit和c-fms,在无细胞试验中IC50分别是10 nM,30 nM,47 nM,84 nM,74 nM,140 nM和146 nM。Pazopanib 可诱导自噬II型细胞死亡。 大剂量询单有大折扣! 今日订购,明日送达,全国免运费!全国免费电话:400-668-6834|Email:info@selleck.cn 产品描述 Pazopanib HCl (GW786034 HCl)是一种新型多靶点抑制剂,作用于VEGFR1,VEGFR2,VEGFR3,PDGFR,FGFR,c-Kit和c-fms,在无细胞试验中IC50分别是10 nM,30 nM,47 nM,84 nM,74 nM,140 nM和146 nM。Pazopanib 可诱导自噬II型细胞死亡。 在HUVEC细胞中,Pazopanib显著抑制VEGF诱导的VEGFR-2的磷酸化,IC50 为8nM [1]。 在所有滑膜肉瘤细胞系中Pazopanib都表现出了生长抑制作用,包括SYO-1和HS-SY-II细胞,这种抑制具有剂量依赖的特性。1µg/mL Pazopanib就可以抑制SYO-1和HS-SY-II细胞增殖,而5µg/mL Pazopanib可以让它们的增殖彻底受阻。 Pazopanib 会诱导滑膜肉瘤细胞周期停留在 G1期从而抑制它的生长。与对照的细胞相比,经过Pazopanib处理过的 SYO-1细胞中的AKTs, GSK-3β, JNKs, p70 S6激酶以及mTOR的磷酸化都受到了抑制[2]。在20 mg/mL 到 22.5 mg/mL的浓度之间,Pazopanib 使RPE细胞的生存能力逐渐下降[3]。 NFPTbGxKdmirYnn0bY9vKG:oIGLJVGsyKGmwIHj1cYFvKEiWLUK5JINmdGy|IHHzd4V{e2WmIHHzJJJmHWldHnvckBqdiCWTlhcJBp[S2rbnT1Z4VlKG6nY4LvdJRwe2m|IHnuZ5Vj[XSnZDDmc5IhOTRidH:gNlQhcHK|IHnuJJBzXOnbnPlJI9nKFOvYXOgcYluXSrYzDhcoQhgi2YQVStSm1MKGK7IHPlcIwhfGm2ZYKg[4xwNWKjc3XkJIx2dWmwZYPj[Y5kSCjc4PhfUwhUUN3ME2wMlg4O87:TR?= MnjTQIEhfGG{Z3X0QUdg[myjbnunJIhzWZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MkKwPVYoRjNzNkKyNFk3RC:jPh?= MlnpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEK5JINmdGy|IHnuJJNmenWvIHPvcpRicW6rbnegcYVlcXWv MXG8ZUB1[XKpZYS9K39jdGGwazegbJJmj1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{MEO4Nkc,OTh4MkCzPFI9N2F- NVjOdY9ySW62aYDyc4xqmW{YYTpeoUh[WO2aYpeJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgbY4he2W{dX2gZ49vfGGrbnnu[{BuWSrdX2= NH3F[YE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJuWRwbnPibU5vdG1wbnnoModwfi9zOE[yNFM5Oid-MUi2NlA{QDJ:L3G+ MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF|N{XQJINmdGy|IHnuJJNmenWvIHPvcpRicW6rbnegcYVlcXWv M4jCdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1mC6wY3LpMo5tdS6waXiu[493NzF6NkKwN|gzLz5zOE[yNFM5OjxxYU6= MXLxTHRUKG:oIIDl[IlifHKrYzDjZY5kXJiY3XscEBtcW6nczD0c{BqGWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IHvdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBkWyucx?= M2X4NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1mC6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6= MkDmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5IhHK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= M2LybVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1mC6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6= MnPSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5IhHK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= M{Hib|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1mC6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6= NHfnSodyUFSVIH;mJJBmGmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBtSCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy= NHjwZmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJuWRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+ NFPQTJpyUFSVIH;mJJBmGmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBtSCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz MXi8ZUB1[XKpZYS9K39jdGGwazegbJJmj1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F- NEkNHNyUFSVIH;mJJBmGmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBtSCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M{SNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1mC6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6= M{jIUZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBkWyucx?= MmjrQIEhfGG{Z3X0QUdg[myjbnunJIhzWZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?= NH:3WGJyUFSVIH;mJJBmGmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBtSCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= NUPMeog2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi= MkXWdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5IhHK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? 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The expression of P-Akt (S473) was detected by western blotting in RKO or HT-29 cells after 20 M pazopanib treatment for indicated times. p-VEGFR2 / p-c-Kit / p-PDGFR / FLT-3 ; PubMed: 19844230 Br J Cancer Inhibition of receptor autophosphorylation by various tyrosine kinase inhibitors. To compare the activities of pazopanib, sunitinib, and sorafenib, we evaluated their inhibitory effects against wild-type VEGFR-2, c-Kit, PDGFR- , and Flt-3 receptors in HUVEC (for VEGFR-2), NCI-H526 (c-Kit), HFF (PDGFR- ), and RS4;11 (Flt-3). Cells were serum-starved overnight and then treated with DMSO or different concentrations of pazopanib, sunitinib, or sorafenib for 2 h. Cells were then stimulated with respective ligands as described in the cellular autophosphorylation section under Materials and Methods. Total receptor was immunoprecipitated using antireceptor antibodies and phosphorylation was detected using anti-pTyr antibody following western blot analysis. YAP/TAZ; PubMed: 26199863 FEBS Open Bio Dasatinib, fluvastatin, and pazopanib inhibited the nuclear localization of YAP and TAZ. MDA-MB-231 cells were treated with inhibitors for 8h and YAP and TAZ were visualized by immunofluorescence. (i) Representative images of immunofluorescence. Bar represents 10 m. Cell viability; PubMed: 27924057 Oncotarget Cell viability was analyzed using Cell Counting Kit-8 at 0, 3, 6, 12 and 24 hours after 1, 5, 10, or 20 M pazopanib treatment in HCT-116 cells. Data represent the mean SEM of four independent experiments. 与没有经过Pazopanib或者仅用10 mg/kg的Pazopanib治疗的小鼠相比,经过30 mg/kg或者100 mg/kg Pazopanib治疗的小鼠其肿瘤症状明显减轻。Pazopanib治疗耐受性良好,各组小鼠之间体重没有发生明显差异[2]。 激酶活性测定: 通过杆状病毒表达系统纯化人源的VEGFR受体激酶1, 2和3的C端催化活性区域的GST融合蛋白,用这些蛋白在384孔微量滴定板上利用均相时间分辨荧光技术(HTRF)对VEGRF1,2和3的酶活性进行分析。首先将10 μL活化的VEGFR2激酶溶液[终浓度为1nM酶溶于0.1 M HEPES,pH 7.5,包含0.1 mg/mL BSA,300 μM DTT]加入10μL底物溶液[终浓度为360 nM多肽(生物素偶联的aminohexyl-EEEEYFELVAKKKK-NH2),75 μM ATP, 10 μM MgCl2],同时加入1 μL 溶解在DMSO中的Pazopanib。滴定板在室温条件下孵育1小时,然后加入20 μL 100 mM 的EDTA终止反应。终止之后,加入20μL HTRF试剂(终浓度为15 nM 链亲和素偶联的别藻蓝素,用0.1 mg/mL BSA, 0.1 M HEPES, pH 7.5稀释的1 nM铕标记的酪氨酸磷酸化抗体)孵育十分钟以上。使用Wallac Victor的时间分辨荧光仪测定665 nM处的荧光值,时间间隔为50 μs。 Incubation Time: 1 小时 Method: 检测经过VEGF处理过的HUVEC细胞中VEGFR2的磷酸化水平。细胞按每板100万到150万个的密度培养在铺有I型胶原蛋白的10 cm平板上,培养基选用 Clonetics EGM-MV。培养24小时后, 将原来的培养基换成含有0.1% BSA和500 g/mL hydrocortisone 的Clonetics EBM 培养基,对细胞进行过夜的血清饥饿处理。然后用不同浓度的 Pazopanib 处理细胞1小时, 随后用10 ng/mL 的VEGF 或者对照物处理细胞10分钟。将细胞溶解在裂解液中。用flk-1的抗体对VEGFR2进行免疫沉淀反应,随后进行SDS-聚丙烯酰胺凝胶电泳,然后用flk-1 或者 antiphosphotyrosine (anti-P-tyr-biotin)的抗体进行蛋白免疫印迹的检测。通过光密度法测量将VEGFR2的磷酸化水平量化并将总的VEGFR2水品标准化。(Only for Reference) Dosages: 0 mg/kg, 10 mg/kg, 30 mg/kg或100 mg/kg Administration: 口服(Only for Reference) 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献): 30% PEG400+0.5% Tween80+5% propylene glycol * 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。 第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方) 药物溶于 μL DMSO溶液(母液浓度 mg/mL,注:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck); 体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。 体内配方配制方法:取 μL DMSO母液,加入 μL Corn oil,混匀澄清。 注意:1. 首先保证母液是澄清的; 2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。 本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为: 质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol) 摩尔浓度计算公式 *在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。 用本工具协助配置特定浓度的溶液,使用的计算公式为: 开始浓度 x 开始体积 = 最终浓度 x 最终体积 稀释公式 稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 ) 在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。. 连续稀释计算器方程NCT03735758 Recruiting Drug: pazopanib or guideline conform chemotherapy Soft Tissue Sarcoma Adult GWT-TUD GmbH November 2 2018 Phase 4NCT03334409 Terminated Drug: Ascorbic Acid|Drug: Pazopanib Hydrochloride Clear Cell Renal Cell Carcinoma|Metastatic Clear Cell Renal Cell Carcinoma|Stage III Renal Cell Cancer AJCC v8|Stage IV Renal Cell Cancer AJCC v7|Unresectable Renal Cell Carcinoma Academic and Community Cancer Research United|National Cancer Institute (NCI) February 16 2018 Phase 2NCT03149120 Withdrawn Biological: Nivolumab|Drug: Pazopanib Soft Tissue Sarcomas NYU Langone Health|Bristol-Myers Squibb August 2017 Phase 2NCT03091465 Completed Metastatic Renal Cell Carcinoma Spanish Oncology Genito-Urinary Group December 20 2016 在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。 操作手册 Potent triple angiokinase inhibitor for VEGFR1/2/3, FGFR1/2/3 and PDGFRα/β with IC50 of 34 nM/13 nM/13 nM, 69 nM/37 nM/108 nM and 59 nM/65 nM. Phase 3. SU5402是一种有效的多靶点受体激酶抑制剂,对VEGFR2,FGFR1,和PDGF-Rβ的IC50分别为20 nM,30 nM,和510 nM。 Erlotinib (OSI-774, CP358774, NSC 718781, Tarceva)是一种EGFR抑制剂,IC50 为 2 nM,对EGFR的敏感性比对人c-Src 或 v-Ab高1000多倍。Erlotinib可诱导自噬。 Bemcentinib (R428, BGB324)是一种Axl抑制剂,IC50为14 nM,作用于Axl比作用于Abl选择性高100倍以上。作用于Axl选择性也比作用于Mer和Tyro3(高50到100倍)及InsR, EGFR, HER2,和PDGFRβ(高100倍以上)高。 Regorafenib (BAY 73-4506, Fluoro-Sorafenib, Resihance, Stivarga) 是一个多靶点抑制剂,作用于VEGFR1,VEGFR2,VEGFR3,PDGFR-β,Kit (c-Kit),RET (c-RET)和Raf-1,在无细胞试验中IC50分别是13 nM,4.2 nM,46 nM,22 nM,7 nM,1.5 nM和2.5 nM。Regorafenib 可诱导自噬。 Features:Regorafenib是一个新的口服多激酶抑制剂。 Axitinib (AG 013736)是一种多靶点抑制剂,作用于 VEGFR1,VEGFR2,VEGFR3,PDGFRβ和c-Kit,在猪主动脉内皮细胞中IC50分别为0.1 nM,0.2 nM,0.1-0.3 nM,1.6 nM和1.7 nM。 Features:Axitinib与目前使用的sorafenib相比,作为二代治疗法,更有效。 Cabozantinib (XL184, BMS-907351)是一种有效的VEGFR2抑制剂,在无细胞试验中IC50为0.035 nM,也能有效抑制c-Met、 Ret、 Kit、Flt-1/3/4、Tie2和AXL,IC50分别为1.3 nM,4 nM,4.6 nM,12 nM/11.3 nM/6 nM,14.3 nM 和 7 nM。Cabozantinib 在结肠癌细胞中可通过AKT/GSK-3β/NF-κB信号通路诱导PUMA依赖的凋亡。 Nintedanib (BIBF 1120, Intedanib, Vargatef, Ofev) 尼达尼布是一种有效的三重血管激酶抑制剂,作用于VEGFR1/2/3, FGFR1/2/3和PDGFRα/β,在无细胞试验中IC50分别为34 nM/13 nM/13 nM, 69 nM/37 nM/108 nM和59 nM/65 nM。Phase 3。 Vandetanib (ZD6474) 是一种有效的 VEGFR2 抑制剂,在无细胞试验中IC50为40 nM。同时,也抑制VEGFR3和EGFR,IC50分别为110 nM 和500 nM。对PDGFRβ, Flt-1, Tie-2 和FGFR1作用效果不大,IC50为 1.1-3.6 μM, 对MEK, CDK2, c-Kit, erbB2, FAK, PDK1, Akt 和 IGF-1R几乎没有作用效果,IC50>10 μM。Vandetanib (ZD6474) 可增加凋亡并通过提高 reactive oxygen species (ROS) 的水平来诱导自噬。 Lenvatinib (E7080)是一种多靶点抑制剂,无细胞试验中,作用于VEGFR2(KDR)/VEGFR3(Flt-4)最有效,IC50为4 nM/5.2 nM,对VEGFR1/Flt-1作用效果稍弱,作用于VEGFR2/3比作用于FGFR1, PDGFRα/β选择性高10倍左右。Lenvatinib (E7080) 也是FGFR1-4、PDGFR、Kit (c-Kit)和RET (c-RET)的抑制剂,并具有强效的抗肿瘤活性。Phase 3。 Features:E7080是口服有效的多靶点激酶抑制剂。 Pazopanib (GW786034) 是一种新型多靶点的VEGFR1,VEGFR2,VEGFR3,PDGFR,FGFR,c-Kit 和 c-Fms/CSF1R抑制剂,无细胞试验中IC50分别为10 nM,30 nM,47 nM,84 nM,74 nM,140 nM 和 146 nM。Pazopanib 可诱导 cathepsin B 的活化和自噬。 Tags: 购买Pazopanib HCl (GW786034 HCl) | Pazopanib HCl (GW786034 HCl)供应商 | 采购Pazopanib HCl (GW786034 HCl) | Pazopanib HCl (GW786034 HCl)价格 | Pazopanib HCl (GW786034 HCl)生产 | 订购Pazopanib HCl (GW786034 HCl) | Pazopanib HCl (GW786034 HCl)代理商

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