Anti-IRS-1 Antibody (Phospho-Ser 312) (OAAI00339)

货号：	OAAI00339
抗体英文名：	Anti-IRS-1 Antibody (Phospho-Ser 312) (OAAI00339)
靶点：	IRS1
应用范围：	WB, IP
宿主：	Rabbit
适应物种：	Human, Rat, Mouse
克隆性：	否
保存条件：	The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity.Avoid repeated freezing thawing cycles.
亚型：	Rabbit IgG
免疫原：	A synthetic peptide corresponding to the epitope ATSPA with a single phosphorylation site Ser 312 of Human IRS-1.
规格：	100 ug

NCBI Gene Id: 3667Swissprot Id: P35568Insulin Receptor SubstRate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substRates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, sμggesting a potential mechanism for insulin resistance in some models of obesity. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00339

Phospho-peptide, non-phospho-peptide

Dot Blot: 1 ?g peptide was blot onto NC membrane A: IRS-1(pS312) B: IRS-1(non phosphorylated) C: Non-related Phosphopeptide) Followed by rabbit antibodies incubation at a 1:1000 dilution: 1: Rabbit anti-IRS-1(pS312) (Cat#OAAI00339) 2: Rabbit anti-IRS-1(Paired S312) Cat# (602-620)

insulin stimulated CHO

Western Blot: The cell lysate derived from insulin stimulated CHO was immunoprecipitated by Rabbit anti IRS-1 (pS312), (Cat#OAAI00339), then immunoprobed by the same antibody at 1:500 (lane 1). Lane 2 is a negative control.

温馨提示：不可用于临床治疗。