Altogen/Whole Cell Lysate/1 mg of FaDu Whole Cell Lysate(Catalog #8930)

Description

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WholeCellLysate,1mg(2mg/ml)

GeneralNotes:
Growthmedia:DMEMsupplementedwith10%FBS

CelllysatewaspreparedbyhomogenizationinRIPAbuffer(25mMTrisHClpH7.6,150mMNaCl,1%NP-40,1%sodiumdeoxycholate,0.1%SDS)supplementedwith5µg/mlofaprotininand5µg/mlofleupeptin.Celldebriswereremovedbycentrifugation(5minutesat12,000RPM).ConcentrationoftotalproteinwasdeterminedusingBCAproteinassay.

StorageInstructions
Shippedat4°C.Upondeliveryaliquot.Storeat-80°C.Avoidfreeze/thawcycle.

Volume
0.5ml

Concentration
1mgat2mg/ml

Purity
WholeCellLysate

AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

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