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celexplorer/FocusClear™/5 ml/FC-101

Introduction
FocusClear™isnon-toxic,readytouseandawater-solubleclearingagentforenhancingthetransparencyofcross-linkingagent-fixedBIOLOGicalspecimensanditsclearingeffectisabletoreachuptoadepthof500micronsbelowthespecimensurface.WiththeuseofFocusClear™tosignificantlyincreasetheeffectivenessoflaserexcitationanddetectionofcolorandfluorescence,obtainedmicroscopicimagequalitywillbegreatlyimproved.Sampleslabeledwithfluorescenceandnon-fluorescencedyesincludinglipophilicdyes,suchasDiI,DiDandNBD-ceramidecanbedirectlytransferredfromwater,buffersolutions,alcohol,DMSO,DMF,andglycerinintoFocusClear™solution.
Highlights
  • Awater-solubleclearingagentthatincreasesthetransparencyofbiologicaltissues.
  • Allowsforvisualizationofinternalobjectsupto500μmbelowthetissuesurface.
  • Increasesdepthofviewbyatleast2XovertrADItionalglycerol-basedmountingmedia.
  • Increasestheefficacyoflaserexcitationandopticalsignaldetectionofeithercolororfluorescence.
  • Suitableforimmunofluorescent-labeledsinglecellsandtissues,insituhybridization,tissueorwhole-mountimmunohistochemistryandfluorescentproteinsamples.

ProductSpecifications

Specification

RefractiveIndex:1.45

pH:11

Application
Animal:brain,kidney,heart,stomach,intestine,liver,lung,pancreas,skinInsect:brain,ventralnervecord,ovaryBiomaterial:collagenmatrixPlant
Clearing
Solitaryuse
Recommended0.5~1mmdepthtissueslice
WithCLARITY*
RecommendedWholeorgan
WithSCALEVIEW**
Notrecommended
ClearingSpeed
Solitaryuse
Within1Hour
WithCLARITY
Days
WithSCALEVIEW
-
ImmunostainingCompatibility
Yes
FluorescentproteinCompatibility
Yes
OrganicdyeCompatibility
Yes
LipophilicdyeCompatibility
Yes
Objectivelens
ObjectivelenswithhighNAandlongWDissuggested,LeicaHCXIRAPOL25Xwater-immersionobjective,NA0.95,WD2.4mm;Zeiss20XW-PlanApochromatobjective,NA1.0,WD2.0mmOlympusXLSLPLN25XSVMPobjective,NA0.9,WD8.0mm
Cat.No.
FC-101
FC-102
FC-103
Format
5ml
50ml
500ml
Storage
4℃~30℃39℉~86℉

MSDSforFocusClear

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