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CatalogNo.:AB0049-500
Price/Unit:EUR450.00
Source:Goat
Quantity/unitsize:1mg
Description:GoatpolyclonaltoGAPDH(glyceraldehyde3-phosphatedehydrogenase).GAPDHcatalyzesanimportantenergy-yieldingstepincarbohydratemetabolism,thereversIBLeoxidativephosphorylationofglyceraldehyde-3-phosphateinthepresenceofinorganicphosphateandnicotinamideadeninedinucleotide(NAD).Theenzymeexistsasatetramerofidenticalchains.Alternativenames:glyceraldehyde3-phosphatedehydrogenase,glyceraldehyde-3-phosphatedehydrogenase,G3PD,GAPD,HGNC:4141,GAPDHantibody.
Form:Polyclonalantibodysuppliedasa200µl(2mg/ml)aliquotinPBS,20%glyceroland0.05%sodiumazide.Thisantibodyisepitope-affinitypurifiedfromgoatantiserum.
Immunogen:Purifiedrecombinantpeptidederivedfromwithinresidues240aatotheC-terminusofhumanGAPDHproducedinE.coli.
Specificity:Detectsabandof37kDabyWesternblotinthefollowinghuman(293A,HMEC-1,U-118,HaCat),rat(TR-iBRB),mouse(AtT-20,Hepa),canine(D17)andmonkey(COS-7)wholecelllysates
Reactivity:Reactsagainsthuman,rat,mouse,zebrafish,canineandmonkeyproteins.
Usage:Westernblot 1:500-1:5,000Immunofluorescence 1:50-1:250Immunohistochemistry(paraffin) 1:200-1:1,000Immunohistochemistry(frozen) 1:200-1:1,000
Reactivitychart
| Sample | WesternBlot | Immuno-fluorescence | Immuno-Histochemistry(p) | Immuno-Histochemistry(f) | human | +++ | +++ | +++ | +++ | rat | +++ | +++ | +++ | +++ | mouse | +++ | +++ | +++ | +++ | zebrafish | +++ | ND | ND | ND | canine | +++ | +++ | +++ | +++ | monkey | +++ | +++ | +++ | +++ |
|---|
Storage:Storeat-20Cforlongtermstorage.Storeat2-8Cforuptoonemonth.
Specialinstructions:Avoidfreeze/thawcycles.
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