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Category | Mouse monoclonal antibody protein G purified lyophilized |
Reconstitution | For reconstitution add 100 µl distilled water |
Isotype | IgG2a |
Antigen | CD14 knock out mouse immunized with recombinant mouse CD14 |
Special note | biG 53 represents an excellent marker for mouse CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage likes cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. |
Characteristic | Recognize LPS binding site at monocytes. Up to 10 µg/ml biG53 inhibit binding of LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry) |
Application | Suitable for immunostaining of CD14 positive cells and inhibition studies, ELISABinds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 |
Storage | Long time storage at –20°C or –80°C |
Quantity | 100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
| IgG-content | 1 mg/ml PBS (determined by mouse IgG-ELISA) |
Download | Material Safety Data Sheet - Proteins and Antibodies |
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