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Category | Protein G purified, lyophilized |
Reconstitution | For reconstitution add 100 µl distilled water. |
Isotype | IgG2a |
Antigen | Monocytes of different species and immunoaffinity purified soluble human CD14 |
Special note | biG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. |
Characteristic | 1 µg/ml inhibits binding of 0.5 µg/ml LPS to CD14, binding epitope amino acid 39-44Cross reacting with CD14 of bovine++, porc++, dog+, horse+, guinea pig+Inhibits binding of LPS to CD14 of porc++, dog+, bovine +, dog+Binding titre at human CD14 transfected CHO-cells: more than 10.000 |
Application | Suitable for Immunostaining of CD14 positive cells (Flow cytometry), ELISA, CD14 inhibition studies, Western Blot |
Storage | Long time storage at –20°C or –80°C |
Quantity | 100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
| IgG-content | 1 mg/ml PBS |
Download | Material Safety Data Sheet - Proteins and Antibodies |
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