Category | Mouse monoclonal antibody protein G purified (lyophilized) |
Reconstitution | reconstitute with 100 µl distilled water |
Isotype | IgG1 |
Antigen | Human monocytes, monocytes of different species and soluble human CD14 |
Special note | biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. |
Characteristic | inhibits binding of LPS to CD14, binding epitope: amino acid 9-13, 39-44, total inhibition of LPS-binding to CD14: 100µg/mlBinding titre at human CD14 transfected CHO-cells: more than 10.000 |
Application | Suitable for immunostaining of CD14 positive cells (Flow cytometry) ELISA, CD14 inhibition studies, Western Blot |
Storage | Long time storage at –20°C or –80°C |
Quantity | 100 µg in PBS without NaN3 or other stabilisation before lyophilisation |
IgG-content | 1 mg/ml PBS |
Download | Material Safety Data Sheet - Proteins and Antibodies |
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