Spectra/Gel makes the concentration of protein sample solutions quick and efficient. Made of polyacrylate-polyalcohol it has the property of expanding as it absorbs water. The high molecular weight polymer can be used with dialysis membranes of molecular weight cut off"s (MWCO) from 100 to 1,000,000 Daltons without contaminating the sample. After dialysis, simply remove the dialysis tubing containing the sample and coat it with Spectra/Gel. Water and smaller molecules diffuse through the membrane and are absorbed by Spectra/Gel Absorbent. Concentration continues unattended until a certain volume of water is removed from the sample. The insoluble characteristic makes Spectra/Gel easy to remove after absorbancy.
The length of time needed for concentration will depend on the final volume, the MWCO, the surface to volume ratio of the membrane tubing and the amount of Spectra/Gel Absorbent. After dialysis in a Spectra/Por Float-A-Lyzer G2 with a 20 kD MWCO, the volume can be reduced from 5 mL to 0.5 mL in 60 minutes with 100 grams of the Spectra/Gel Absorbent.
Easy-to-use dialysis products such as Spectra/Por Float-A-Lyzer G2 can be used with Spectra/Gel to concentrate samples.
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