Lipidure-CM5206 (White powder), 1g amsbio AMS.52000011GB1G 4,900 其他试剂

Spheroid cell culture is typically based on the spontaneous formation of an aggregate of cells in an environment where cell-cell interactions dominate over cell-substrate interaction. This can be achieved by using low-attachment cell culture conditions. Lipidure-COAT platesand dishes are a top of the range solution for spheroid formation, with the Lipidure coating providing a superior low-attachment solution for the formation of single spheroids in each well of multi-well plates. Using Lipidure-COAT dishes, it is possible to undertake large scale assays to provide sufficient materials for applications such as Western blotting or gene expression microarrays.Spheroid formation using LIPIDURE® has already been demonstrated for the following cell types: human ES, mouse ES, human iPS, mouse iPS, NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines. A single spheroid is generated in the Lipidure-COAT well, while numerous satellite spheroids are found in the competitor’s plates. This phenomenon shows that a lot of cells adhered to the competitor’s \"low-adhesion” surface. Hep G2 spheroids were formed for 5 days. Cell viability was determined by WST assay after further 2 days incubation with or without Mitomycin-C