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Ursabioscience/Dual VpH™

Description

DualVpH™isawatersolubledualpHandviscosityprobe,excitation300-400nm,andaviscositydependentemissioncenteredaround400nm.Thequantumyieldislowinaqueousmedia(≈1cP),butincreasesastheviscosityincreasesmakingitanidealviscositycontrastprobe.

DualVpH™issolddryandissimplyreconstitutedbyaddingwater/MeOH/EtOHand/orbuffer.OnevialofourViscosityProbewilltypicallyallowfor10measurementstobeundertaken: 50µLofwaterorMeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3,typicallygiveanopticaldensity>0.1at340nm(inspectroscopicMeOH).

Foroptimumperformance,probevialsshouldbestoredat-2to-8°Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.

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