产品资料

Stemgent/Sox2 (SRY-box 2) Antibody | Pluripotency Transcription Factor/09-0024/100 μl

ImmunocytochemistryanalysisofSox2onR1mouseEScells.CellswerestainedwithSox2Antibody(AffinityPurified)usinga1:100dilutionfollowedbyasecondaryCy™3conjugatedantibody(red).DAPIstainingwasperformedtovisualizenuclei(blue).

ProductOverview

Sox2,alsoknownasSRY(sexdeterminingregionY)-box2,isatranscriptionfactoressentialformaintainingself-renewalofundifferentiatedembryonicstem(ES)cells.ChromatinimmunoprecipitationexperimentshaveshownthatSox2andOct4bindtothousandsofgeneregulatorysites,highlightingtheimportanceofthesetranscriptionfactorsinearlyembryonicdevelopment.TheknockdownofSox2geneexpressionbysiRNAinhumanEScellsresultsinlossoftheundifferentiatedstemcellstate.Sox2isoneofthekeytranscriptionfactorsusedtoreprogrammouseandhumanfibroblaststoapluripotentstate.TheSox2Antibody(AffinityPurified)wasscreenedonhumanandmouseEScellsusingimmunocytochemistryandflowcytometryandselectedasthebestantibodyavailableforresearchersneedingtodemonstratepluripotency.

ProductSpecifications

Size

100μl

Concentration

0.5mg/ml

Clone

Polyclonal

Isotype

RabbitIgG

Immunogen

Recombinantproteinfragmentcontainingasequencecorrespondingtoaregionwithinaminoacid45and261ofhumanSox2

Reactivity

Mouse,Human

Preparation

Thisantibodywaspurifiedbyantigenaffinitychromatography.

Formulation

Phosphate-bufferedsolution,pH7.2,1%BSA,and0.1%sodiumazide

StorageandStABIlity

Storeat4˚Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistrystainingtoensureproductquality.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryanda1:5dilutionforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.

SpecificationSheets

09-0024SpecificationsSheet

SafetyDataSheets

09-0024SafetyDataSheet

Protocols

Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
Protocol:Immunocytochemistry

References

Rodda,D.J.,Chew,J.L.,Lim,L.H.,Loh,Y.H.,Wang,B.,Ng,H.H.,andRobson,P.(2005)TranscriptionalregulationofNanogbyOCT4andSOX2.JBiolChem.280:24731-24737.
Boyer,L.A.,Lee,T.I.,Cole,M.F.,Johnstone,S.E.,Levine,S.S.,Zucker,J.P.,Guenther,M.G.,Kumar,R.M.,Murray,H.L.,Jenner,R.G.,Gifford,D.K.,Melton,D.A.,Jaenisch,R.,andYoung,R.A.(2005)Coretranscriptionalregulatorycircuitryinhumanembryonicstemcells.Cell122:947-956.
Fong,H.,Hohenstein,K.A.,andDonovan,P.J.(2008)Regulationofself-renewalandpluripotencybySox2inhumanembryonicstemcells.StemCell26:1931-1938.
Takahashi,K.,andYamanaka,S.(2006).Inductionofpluripotentstemcellsfrommouseembryonicandadultfibroblastculturesbydefinedfactors.Cell126,663-676.
Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
Park,I.H.,Arora,N.,Huo,H.,Maherali,N.,Ahfeldt,T.,Shimamura,A.,Lensch,M.W.,Cowan,C.,Hochedlinger,K.,andDaley,G.Q.(2008)Disease-specificinducedpluripotentstemcells.Cell134:877-886.

AdditionalPublications

PaullD;SevillaA;ZhouH;HahnAK;KimH;NapolitanoC;TsankovA;ShangL;KrumholzK;JagaseesanP;WoodardCM;SunB;VilbouxT;ZimmerM;ForeroE;MoroziewiczDN;MartinezH;MalicdanMCV;WeissKA;VensandLB;DusenberryCR;PolusH;SyKTL;KahlerDJ;GahlWA;SolomonSL;ChangS;MeissnerA;EgganK;NoggleSA."Automated,high-throughputderivation,characterizationanddifferentiationofinducedpluripotentstemcells."NaureMethods12:885(2015)
HanF;WangW;CHenB;CHenC;LISen;LuX;DuanJ;ZhangY;ZhangYA;GuoW;LiG."Humaninducedpluripotentstemcell-derivedneuronsimprovemotorasymmetryina6-hydroxydopamine-inducedratmodelofParkinson"sdisease."Cytotherapy17:665(2015)

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