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Stemgent/Goat anti-Rabbit IgG Antibody (DyLight 488) | Secondary Antibody/09-0034/200 μl

09-0034_figure1
ImmunocytochemicalanalysisofOct4onH1humanEScells.CellswerefixedandstainedwithOct4Antibodyusinga1:100dilutionfollowedbyGoatanti-RabbitIgGAntibody(DyLight488)(green).DAPIstainingwasperformedtovisualizenuclei(blue).
09-0034_figure2
FlowcytometricanalysisofOct4onH1humanEScells.PurplehistogramrepresentsOct4AntibodyfollowedbyGoatanti-RabbitIgGAntibodyandopenhistogramrepresentsisotypecontrolatthesameconcentration.

ProductOverview

TheGoatanti-RabbitIgGAntibody(DyLight488)reactswithrabbitIgGandthelightchainsofotherrabbitimmunoglobulins.Crossreactivityagainstnon-immunoglobulinserumproteinshasnotbeendetected.

ProductSpecifications

Size

200μl

Concentration

0.5mg/ml

Clone

Polyclonal

Isotype

GoatIgG

Reactivity

Rabbit,Human

Preparation

ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight488underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.

Formulation

Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide

StorageandStablilty

Storeat4°Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryanda1:20dilutionforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.

SpecificationSheets

  • 09-0034SpecificationsSheet

SafetyDataSheets

  • 09-0034SafetyDataSheet

Protocols

  • Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
  • Protocol:Immunocytochemistry
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