产品资料

Stemgent/StainAlive SSEA-1 Antibody (DyLight 550) | Stage-specific Embryonic Antigen 1/09-0095/100 μl

ICCanalysisofSSEA-1AntibodyonR1mouseEScellsat10Xmagnification.[A]Phasecontrastviewofcellsinculture,[B]samefieldofviewafterstainingwithStainAliveSSEA-1Antibody(DyLight550)usinga1:100dilution.

ProductOverview

Stage-specificembryonicantigen-1(SSEA-1),alsoknownasCD15,isanantigenicepitopedefinedasaLewisXcarbohydratestructure.Itisexpressedonmurineembryonalcarcinoma(EC)cells,murineembryonicstem(ES)cells,andmurineandhumangermcells.SSEA-1iswidelyusedasapositivesurfaceMarkerformouseundifferentiatedstemcellsandanegativesurfacemarkerforhumanundifferentiatedstemcells.Expressionisdown-regulatedfollowingdifferentiationofmurineECandEScells,whilethedifferentiationofhumanECandEScellsisaccompaniedbyanincreaseinSSEA-1expression.SSEA-1isassociatedwithcelladhesion,migration,anddifferentiation.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andexpressionofpluripotencymarkersdoesnotchangeafterstainingwiththisantibody.

ProductSpecifications

Size

100μl

Concentration

0.5mg/ml

Clone

MC-480

Isotype

MouseIgM,κ

Immunogen

MouseF9teratocarcinomastemcells(X-irrADIated)

Reactivity

Mouse,Human

Preparation

ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight550underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.Theformulationis0.2μmfiltersterilizedandfreefrombacteria,fungi,mycoplasma,andvirus.

Formulation

Phosphate-bufferedsolution,pH7.2,containingnopreservative

StorageandStABIlity

Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistrytoensureproductquality.
Endotoxinlevelislessthan0.1EU/μg(0.01ng/μg)oftheproteinasdeterminedbytheLALassay.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.Foranapplicationspecificprotocol,pleasereferenceProtocol:ImmunocytochemistryofLiveCellsonlineatwww.Stemgent.com/support/protocols.

SpecificationSheets

09-0095SpecificationsSheet

SafetyDataSheets

09-0095SafetyDataSheet

Protocols

GeneralProtocolforICCStainingofLiveCells

References

Brambrink,T.,Foreman,R.,Welstead,G.G.,Lengner,C.J.,Wernig,M.,Suh,H.,andJaenisch,R.(2008)Sequentialexpressionofpluripotencymarkersduringdirectreprogrammingofmousesomaticcells.CellStemCell2:151-159.
Fox,N.,Damjanov,I.,Martinez-Hernandez,A.,Knowles,B.B.,andSolter,D.(1981)Immunohistochemicallocalizationoftheearlyembryonicantigen(SSEA-1)inpostimplantationmouseembryosandfetalandadulttissues.DevBiol83:391-398.
Andrews,P.W.,Goodfellow,P.N.,Shevinsky,L.,Bronson,D.L.,andKnowles,B.B.(1982)Cellsurfaceantigensofaclonalhumanembryoniccarcinomacellline:morphologicalandantigenicdifferentiationinculture.Int.J.Cancer29:523-531.
Draper,J.S.,Pigott,C.,Thomson,J.A.,andAndrews,P.W.(2002)Surfaceantigensofhumanembryonicstemcells:changesupondifferentiationinculture.J.Anat.200:249-258.
Cui,L.,Johkura,K.,Yue,F.,Ogiwara,N.,Okouchi,Y.,Asanuma,K.,andSasaki,K.(2004)SpatialdistributionandinitialchangesofSSEA-1andothercelladhesion-relatedmoleculesonmouseembryonicstemcellsbeforeandduringdifferentiation.JHistochemCytochem52:1447-1457.

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