产品资料

Stemgent/Alkaline Phosphatase Staining Kit/00-0009/50 assays

StainingiPScellsforpluripotency

ProductOverview

Thisproducthasbeendiscontinued. PleaseseeAlkalinePhosphataseStainingKitII(00-0055)forareplacement.

Theundifferentiatedstateofembryonicstem(ES)andinducedpluripotentstem(iPS)cellscanbecharacterizedbyahighlevelofalkalinephosphatase(AP)expressionwhich,alongwiththeexpressionofothersurfaceMarkers,indicatesundifferentiatedcellswiththepotentialtoself-renew.APisahydrolaseenzymeresponsIBLefordephosphorylatingmoleculessuchasnucleotides,proteins,andalkaloidsunderalkalineconditions.WhenfixedESoriPScellsarestainedusingtheAPStainingKit,undifferentiatedcellsappearredorpurple,whereasdifferentiatedcellsappearcolorless.

ProductSpecifications

Size

50assays

KitComponents

FixSolution:25ml
APStainingSolutionA:10ml
APStainingSolutionB:10ml

StorageandStABIlity

Storeat4°C.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

TheAPStainingKitisfunctionallytestedonhumanandmouseEScellstoensureproductquality.

SpecificationSheets

00-0009SpecificationsSheet

SafetyDataSheets

09-0016SafetyDataSheet
00-0009SafetyDataSheet
09-0017SafetyDataSheet
09-0018SafetyDataSheet

Protocols

Protocol:AlkalinePhosphataseStainingKit

References

Pease,S.,Braghetta,P.,Gearing,D.,Grail,D.,andWilliams,R.L.(1990)Isolationofembryonicstem(ES)cellsinmediasupplementedwithrecombinantleukemiainhibitoryfactor(LIF).DevBiol.141:344-352.
Smith,A.G.,Nichols,J.,Robertson,M.,andRathjen,P.D.(1992)Differentiationinhibitingactivity(DIA/LIF)andmousedevelopment.Dev.Biol.151:339-351.
Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.

AdditionalPublications

JosowitzR,LuJ,FalceC,D’SouzaSL,WuM,CohenN,DuboisNC;ZhaoY;SobieEA;FishmanGI;GelbBD.Identificationandpurificationofhumaninducedpluripotentstemcell-derivedatrial-likecardiomyocytesbasedonsarcolipinexpression.PLoSONE9(7):e101316(2014)

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒