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Astartebio/CMV Specific T Cells, HLA-B*35 restricted/1048-2996NV15/1-2M cells/vial

ProductDetails

BloodType:OPos
Age:29
Gender:Male
Ethnicity:Caucasian
HLA-A:01,03
HLA-B:08,35
HLA-C:04,07
HLA-DRβ1:15,01

AdditionalDonorInformation:

AntigenSpecificInterferon-γSecretion

AntigenIFN-γConcentration(pg/mL)
Tcellsonlycontrols6.4
plusAPC13.9
plusAPC+HLA-B35EBNA15.7
75,000Tcellswereplatedina96-wellround-bottomplatealoneorinthepresenceof10,000APCs(B-LCLsexpressingHLA-B35andHLA-A2)aloneorinthepresenceof1μg/mLHLA-B35restrictedEBNApeptide(HPVGEADYFEY),HLA-B35restrictedCMVpp65peptide(IPSINVHHY)orHLA-A2restrictedCMVpp65peptide(NLVPMVATV).Afteranovernight(18-24h)incubationperiod,supernatantwascollectedfromeachwell.IFN-γconcentrationwasanalyzedusingtheMesoScaleDiscoveryIFN-γassay.

Healthyyoungdonortakingnomedications.HeisCMVSEROpositiveandFcgammaRIIIaF/F.

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