Keyfeatures:
Cat.No: | Description | |
---|---|---|
GSPSSD2-001 | 1600u | GspSSD2.0DNApolymerase@8u/µl |
GSPSSD2-002 | 8000u | GspSSD2.0DNApolymerase@8u/µl |
GSPSSD2-002HC | 8000u | GspSSD2.0DNApolymerase@100u/µl |
GSPSSD2-003HC | 40000u | GspSSD2.0DNApolymerase@100u/µl |
iBuffer5-001 | 2x1.5mliBuffer5 | |
iBuffer5-002 | 15mliBuffer5 |
LAMPreactions(25µl):8UsuppliedDNAPol,1Xmanufacturerssuppliedreactionbuffer(‘GspSSD2.0’–iBuffer5,‘N’–IsoPolII,‘L’–BufferC),dNTPs/MgSO4andBetaine–aspermanufacturersrecommendedconcentrations,100-10,000copiesHumangDNA(Sigma),1XHumanVKORC1primermix–designedusingLAMPDesigner™(0.8µMFIP5’-CTGTTGCACCTACCCTTCTGGAGCAGGAGAGGGAAATATCA-3’,0.8µMBIP5’-GAAGTCAAGCAAGAGAAGACCTGAAAACTCCTGACCTCAAGTGA-3’,0.2µMF35’-ACCATTATTCTGTCTACCACAC-3’,0.2µMB35’-GACAGGGTTTCACCATGTT-3’,0.4µMLoopF5’-TCTCTTCCTCTGGCGTCT-3’,0.4µMLoopB5’-CTCACGCCTATAATCCTAGCATT-3’),3.75ngfluorescentintercalatingdye(V13-01184DyomicsGmbH).‘GspSSD2.0’,‘N’and‘L’reactionswererunaspermanufacturer’sinstructionsat65ºC,65ºCand70ºCrespectivelyonaGenie®II.A:Real-timeamplificationplots.B:Time-to-thresholdvalues(themaximalrateofchangeinfluorescenceovertime)reportedingraphicalformatforsimplecomparison.
LAMPreactions(25µl):8UsuppliedDNAPol,1Xmanufacturerssuppliedreactionbuffer(‘GspSSD2.0’–iBuffer5,‘N’–IsoPolII,‘L’–BufferC),dNTPs/MgSO4andBetaine–aspermanufacturersrecommendedconcentrations,0.1-10ngMS2RNA(Roche),1XMS2primermixdesignedusingLAMPDesigner™(0.8µMFIP5’-TCGCAAGCGAACCATCTACGTCCGTTGGTATAGACCTGAA-3’,0.8µMBIP5’-CGATAGACTTATCGTCTGCATCCGGAGATATGAATATAGCTCTGGTGG-3’,0.2µMF35’-TGTAAGGAGCCTGATATGAATATG-3’,0.2µMB3-TAGTGTGAGCGGATACGAT-3’,0.4µMLoopF5’-GCCAGACGCTGGTTGAT-3’,0.4µMLoopB5’-GATCGCCTGGTGTGGAG-3’),3.75ngfluorescentintercalatingdye(V13-01184DyomicsGmbH).‘GspSSD2.0’,‘N’and‘L’reactionswererunaspermanufacturer’sinstructionsat65ºC,65ºCand70ºCrespectivelyonaGenie®II+/-0.5UAMV-RT(OptiGene).A:Real-timeamplificationplots.B:Time-to-thresholdvalues(themaximalrateofchangeinfluorescenceovertime)reportedingraphicalformatforsimplecomparison.
英国OptiGene Limited 公司位于英国西苏亚克斯郡,长期以来专业生产光学及热力学设备,公司研发生产的Genie系列等温扩增荧光检测系统和配套试剂,使核酸检测更准确、快速,并可通过便携式设备完成.
OptiGene Limited was formed in 2008 to develop and deliver advanced molecular diagnostics solutions for applications across a range of market sectors. The company is privately owned and is based in Horsham in the South of England, close to Gatwick Airport and within an hour of Heathrow Airport and the centre of London. OptiGene was established with the primary goal of providing the highest quality instrumentation and performance-leading reagents to support isothermal amplification of DNA and RNA. The market pull for this venture came from the growing demands around the world for field testing, rapid results and point-of-care diagnostics.
OptiGene has developed innovative products that support sensitive and specific detection of bacteria and viruses for use in the fields of plant health, food safety, veterinary medicine, environmental monitoring and healthcare. Bio-defence and forensics are also key markets that OptiGene addresses, either directly or through its growing network of international distributors. The company has exploited its expertise in both instrument design and enzymology to develop a sophisticated open platform that will support all isothermal amplification methods. Ultra-sensitive molecular detection that has been constrained to laboratory use by highly-qualified personnel and taking hours to complete can now be deployed to point of application and run with very little training, producing results in single minutes.
The Genie II instrument is the flagship product of OptiGene and has been in full production since the spring of 2011. Genie II is now found in a variety of diagnostic settings around the world. It is supported by specially-designed plastic strips that have lockable caps and that are easy to handle. Enzymes are available in the form of several master mixes that feature combinations of high-speed operation, inherent RT activity and thermostability. A continuous programme of development is maintained at OptiGene in order to satisfy the evolving demands of its customers and the wider market. New instruments are planned that will include a smaller device that will suit on-site use and more sophisticated optical arrangements that can support multiplexed assays. Improvements in the company’s enzymes are on-going with faster and more stable master mixes planned for release. OptiGene can also offer to develop variants of its products on behalf of OEM customers and these can adopt the customer’s corporate branding and include special features.
RoHS Compliance
OptiGene Limited declares that, to the best of our knowledge, all electrical and electronic equipment (EEE) sold by the company are in compliance with Directive 2011/65/EU of the European Parliament and of the Council of 8 June 2011 on the restriction of the use of certain hazardous substances in electrical and electronic equipment (also known as “RoHS2”).
REACh Statement
OptiGene Limited also declares that no products sold by the company are known to contain any of the substances of very high concern (SVHC) as intentionally added components with regards to the European Regulation No. 1907/2006 (Registration, Evaluation, Authorisation & restriction of Chemicals).
OptiGene Limited was formed in 2008 to develop and deliver advanced molecular diagnostics solutions for applications across a range of market sectors. The company is privately owned and is based in Horsham in the South of England, close to Gatwick Airport and within an hour of Heathrow Airport and the centre of London. OptiGene was established with the primary goal of providing the highest quality instrumentation and performance-leading reagents to support isothermal amplification of DNA and RNA. The market pull for this venture came from the growing demands around the world for field testing, rapid results and point-of-care diagnostics.
OptiGene has developed innovative products that support sensitive and specific detection of bacteria and viruses for use in the fields of plant health, food safety, veterinary medicine, environmental monitoring and healthcare. Bio-defence and forensics are also key markets that OptiGene addresses, either directly or through its growing network of international distributors. The company has exploited its expertise in both instrument design and enzymology to develop a sophisticated open platform that will support all isothermal amplification methods. Ultra-sensitive molecular detection that has been constrained to laboratory use by highly-qualified personnel and taking hours to complete can now be deployed to point of application and run with very little training, producing results in single minutes.
The Genie II instrument is the flagship product of OptiGene and has been in full production since the spring of 2011. Genie II is now found in a variety of diagnostic settings around the world. It is supported by specially-designed plastic strips that have lockable caps and that are easy to handle. Enzymes are available in the form of several master mixes that feature combinations of high-speed operation, inherent RT activity and thermostability. A continuous programme of development is maintained at OptiGene in order to satisfy the evolving demands of its customers and the wider market. New instruments are planned that will include a smaller device that will suit on-site use and more sophisticated optical arrangements that can support multiplexed assays. Improvements in the company’s enzymes are on-going with faster and more stable master mixes planned for release. OptiGene can also offer to develop variants of its products on behalf of OEM customers and these can adopt the customer’s corporate branding and include special features.
RoHS Compliance
OptiGene Limited declares that, to the best of our knowledge, all electrical and electronic equipment (EEE) sold by the company are in compliance with Directive 2011/65/EU of the European Parliament and of the Council of 8 June 2011 on the restriction of the use of certain hazardous substances in electrical and electronic equipment (also known as “RoHS2”).
REACh Statement
OptiGene Limited also declares that no products sold by the company are known to contain any of the substances of very high concern (SVHC) as intentionally added components with regards to the European Regulation No. 1907/2006 (Registration, Evaluation, Authorisation & restriction of Chemicals).
OptiGene Limited is pleased to announce that the WiFi in Genie III...
OptiGene are pleased to announce new kits available to order: ISO-001-RT400 – Isothermal...
OptiGene Press Release – 2 April 2020 UK company OptiGene has developed...
New kits now available for Flavescence doree: PK-F.doree-050W – Flavescence doree assay...
There is a new software updates available for Genie® II Mark II...
OptiGene Limited is pleased to announce that the WiFi in Genie III...
OptiGene are pleased to announce new kits available to order: ISO-001-RT400 – Isothermal...
OptiGene Press Release – 2 April 2020 UK company OptiGene has developed...
New kits now available for Flavescence doree: PK-F.doree-050W – Flavescence doree assay...
OptiGene will be at APS 2020 in Denver, Colorado
苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!