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OptiGene/GspSSD Isothermal Mastermix (ISO-001)/GspSSD Isothermal Master Mix - 400 reactions/ISO-001

OptiGene/GspSSD Isothermal Mastermix (ISO-001)/GspSSD Isothermal Master Mix - 400 reactions/ISO-001
  • OptiGene/GspSSD Isothermal Mastermix (ISO-001)/GspSSD Isothermal Master Mix - 400 reactions/ISO-001
商品介绍
GspSSD2.0LFDNAPolymerase
  • WORLD’SfastestLAMPandRT-LAMPenzyme.
  • Optigene’sengineeredDNApolymeraseforthefastestisothermalamplificationofDNAandRNA.
  • ContainsourproprietarynovelThermostableGspSSDLFDNAPolymerase;furtherengineeredthroughin-silicodesign.
  • Nativereverse-transcriptaseactivityenablesrapidsingleenzymeRT-LAMP.
  • Keyfeatures:

  • FastestamplificationofDNAandRNA.
  • Superiorstrand-displacementactivity
  • 65ºCtemperatureoptimum
  • 5’-3’exonucleaseminus
  • 3’-5’exonucleaseminus
  • Suppliedwith10XiBuffer5
  • Cat.No: Description
    GSPSSD2-001 1600u GspSSD2.0DNApolymerase@8u/µl
    GSPSSD2-002 8000u GspSSD2.0DNApolymerase@8u/µl
    GSPSSD2-002HC 8000u GspSSD2.0DNApolymerase@100u/µl
    GSPSSD2-003HC 40000u GspSSD2.0DNApolymerase@100u/µl
    iBuffer5-001 2x1.5mliBuffer5
    iBuffer5-002 15mliBuffer5
    LAMP(HumangDNAtemplate):Time-to-thresholdcomparison:GspSSD2.0vscompetitor‘N’(LAMPPol3.0)andcompetitor‘L’(thermostableLAMPPol)

    gspssd2.0 dna a

    LAMPreactions(25µl):8UsuppliedDNAPol,1Xmanufacturerssuppliedreactionbuffer(‘GspSSD2.0’–iBuffer5,‘N’–IsoPolII,‘L’–BufferC),dNTPs/MgSO4andBetaine–aspermanufacturersrecommendedconcentrations,100-10,000copiesHumangDNA(Sigma),1XHumanVKORC1primermix–designedusingLAMPDesigner™(0.8µMFIP5’-CTGTTGCACCTACCCTTCTGGAGCAGGAGAGGGAAATATCA-3’,0.8µMBIP5’-GAAGTCAAGCAAGAGAAGACCTGAAAACTCCTGACCTCAAGTGA-3’,0.2µMF35’-ACCATTATTCTGTCTACCACAC-3’,0.2µMB35’-GACAGGGTTTCACCATGTT-3’,0.4µMLoopF5’-TCTCTTCCTCTGGCGTCT-3’,0.4µMLoopB5’-CTCACGCCTATAATCCTAGCATT-3’),3.75ngfluorescentintercalatingdye(V13-01184DyomicsGmbH).‘GspSSD2.0’,‘N’and‘L’reactionswererunaspermanufacturer’sinstructionsat65ºC,65ºCand70ºCrespectivelyonaGenie®II.A:Real-timeamplificationplots.B:Time-to-thresholdvalues(themaximalrateofchangeinfluorescenceovertime)reportedingraphicalformatforsimplecomparison.

    RT-LAMP(MS2RNAtemplate):Time-to-thresholdcomparison:GspSSD2.0vscompetitor‘N’(LAMPPol3.0)andcompetitor‘L’(thermostableLAMPPol)

    gspssd2.0 dna b

    LAMPreactions(25µl):8UsuppliedDNAPol,1Xmanufacturerssuppliedreactionbuffer(‘GspSSD2.0’–iBuffer5,‘N’–IsoPolII,‘L’–BufferC),dNTPs/MgSO4andBetaine–aspermanufacturersrecommendedconcentrations,0.1-10ngMS2RNA(Roche),1XMS2primermixdesignedusingLAMPDesigner™(0.8µMFIP5’-TCGCAAGCGAACCATCTACGTCCGTTGGTATAGACCTGAA-3’,0.8µMBIP5’-CGATAGACTTATCGTCTGCATCCGGAGATATGAATATAGCTCTGGTGG-3’,0.2µMF35’-TGTAAGGAGCCTGATATGAATATG-3’,0.2µMB3-TAGTGTGAGCGGATACGAT-3’,0.4µMLoopF5’-GCCAGACGCTGGTTGAT-3’,0.4µMLoopB5’-GATCGCCTGGTGTGGAG-3’),3.75ngfluorescentintercalatingdye(V13-01184DyomicsGmbH).‘GspSSD2.0’,‘N’and‘L’reactionswererunaspermanufacturer’sinstructionsat65ºC,65ºCand70ºCrespectivelyonaGenie®II+/-0.5UAMV-RT(OptiGene).A:Real-timeamplificationplots.B:Time-to-thresholdvalues(themaximalrateofchangeinfluorescenceovertime)reportedingraphicalformatforsimplecomparison.

    品牌介绍

    英国OptiGene Limited 公司位于英国西苏亚克斯郡,长期以来专业生产光学及热力学设备,公司研发生产的Genie系列等温扩增荧光检测系统和配套试剂,使核酸检测更准确、快速,并可通过便携式设备完成.

    OptiGene Limited was formed in 2008 to develop and deliver advanced molecular diagnostics solutions for applications across a range of market sectors.  The company is privately owned and is based in Horsham in the South of England, close to Gatwick Airport and within an hour of Heathrow Airport and the centre of London.  OptiGene was established with the primary goal of providing the highest quality instrumentation and performance-leading reagents to support isothermal amplification of DNA and RNA.  The market pull for this venture came from the growing demands around the world for field testing, rapid results and point-of-care diagnostics.

    OptiGene has developed innovative products that support sensitive and specific detection of bacteria and viruses for use in the fields of plant health, food safety, veterinary medicine, environmental monitoring and healthcare.  Bio-defence and forensics are also key markets that OptiGene addresses, either directly or through its growing network of international distributors.  The company has exploited its expertise in both instrument design and enzymology to develop a sophisticated open platform that will support all isothermal amplification methods.  Ultra-sensitive molecular detection that has been constrained to laboratory use by highly-qualified personnel and taking hours to complete can now be deployed to point of application and run with very little training, producing results in single minutes.

    The Genie II instrument is the flagship product of OptiGene and has been in full production since the spring of 2011.  Genie II is now found in a variety of diagnostic settings around the world.  It is supported by specially-designed plastic strips that have lockable caps and that are easy to handle.  Enzymes are available in the form of several master mixes that feature combinations of high-speed operation, inherent RT activity and thermostability.  A continuous programme of development is maintained at OptiGene in order to satisfy the evolving demands of its customers and the wider market.  New instruments are planned that will include a smaller device that will suit on-site use and more sophisticated optical arrangements that can support multiplexed assays.  Improvements in the company’s enzymes are on-going with faster and more stable master mixes planned for release.  OptiGene can also offer to develop variants of its products on behalf of OEM customers and these can adopt the customer’s corporate branding and include special features.

    RoHS Compliance

    OptiGene Limited declares that, to the best of our knowledge, all electrical and electronic equipment (EEE) sold by the company are in compliance with Directive 2011/65/EU of the European Parliament and of the Council of 8 June 2011 on the restriction of the use of certain hazardous substances in electrical and electronic equipment (also known as “RoHS2”).

    REACh Statement

    OptiGene Limited also declares that no products sold by the company are known to contain any of the substances of very high concern (SVHC) as intentionally added components with regards to the European Regulation No. 1907/2006 (Registration, Evaluation, Authorisation & restriction of Chemicals).


    About Us

    OptiGene Limited was formed in 2008 to develop and deliver advanced molecular diagnostics solutions for applications across a range of market sectors.  The company is privately owned and is based in Horsham in the South of England, close to Gatwick Airport and within an hour of Heathrow Airport and the centre of London.  OptiGene was established with the primary goal of providing the highest quality instrumentation and performance-leading reagents to support isothermal amplification of DNA and RNA.  The market pull for this venture came from the growing demands around the world for field testing, rapid results and point-of-care diagnostics.

    OptiGene has developed innovative products that support sensitive and specific detection of bacteria and viruses for use in the fields of plant health, food safety, veterinary medicine, environmental monitoring and healthcare.  Bio-defence and forensics are also key markets that OptiGene addresses, either directly or through its growing network of international distributors.  The company has exploited its expertise in both instrument design and enzymology to develop a sophisticated open platform that will support all isothermal amplification methods.  Ultra-sensitive molecular detection that has been constrained to laboratory use by highly-qualified personnel and taking hours to complete can now be deployed to point of application and run with very little training, producing results in single minutes.

    The Genie II instrument is the flagship product of OptiGene and has been in full production since the spring of 2011.  Genie II is now found in a variety of diagnostic settings around the world.  It is supported by specially-designed plastic strips that have lockable caps and that are easy to handle.  Enzymes are available in the form of several master mixes that feature combinations of high-speed operation, inherent RT activity and thermostability.  A continuous programme of development is maintained at OptiGene in order to satisfy the evolving demands of its customers and the wider market.  New instruments are planned that will include a smaller device that will suit on-site use and more sophisticated optical arrangements that can support multiplexed assays.  Improvements in the company’s enzymes are on-going with faster and more stable master mixes planned for release.  OptiGene can also offer to develop variants of its products on behalf of OEM customers and these can adopt the customer’s corporate branding and include special features.

    RoHS Compliance
    OptiGene Limited declares that, to the best of our knowledge, all electrical and electronic equipment (EEE) sold by the company are in compliance with Directive 2011/65/EU of the European Parliament and of the Council of 8 June 2011 on the restriction of the use of certain hazardous substances in electrical and electronic equipment (also known as “RoHS2”).

    REACh Statement
    OptiGene Limited also declares that no products sold by the company are known to contain any of the substances of very high concern (SVHC) as intentionally added components with regards to the European Regulation No. 1907/2006 (Registration, Evaluation, Authorisation & restriction of Chemicals).

    Tell Me More

    News

    Videos

    • Ash dieback New device tests trees for disease
    • Campylobacter Test Kit Demonstration
    • Plant Testing Kit
    • Food Authenticity Testing Kit
    • Ash Die back testing with Genie II
    • Ash dieback New device tests trees for disease
    • Campylobacter Test Kit Demonstration

    Upcoming Events...

    • APS 2020
      Denver

      08/08/2020 - 12/08/2020

      OptiGene will be at APS 2020 in Denver, Colorado

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