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Biohelix/T7 Gp4A Helicase/H0250S/50 µg

Description:

  


TheT7Gp4AHelicaseexhibitsa5’to3’helicaseactivityaswellasprimaseactivity.Itishighlyprocessive,travelinganaverageof75kbofsinglestrandedDNAbeforedissociating.

SUPPLIEDWITH:
10XGp4AReactionBuffer

SuppliedbyUsers:dTTP

      

Source:

  

PurifiedfromanE.colistrainthatoverexpressesthegp4AgeneisolatedfromtheT7phage.      

Reactioncondition:

  


1XGp4AReactionBuffer:
35mMTris–acetate,pH7.5
11mMmagnesiumacetate
5mMDTT
0.01%TritonX-100

Supplementwith2mMdTTP.
Incubateat25-37ºC.

NOTE:
TheconcentrationofdTTPshouldbeoptimizedforeachspecificapplication.
Fortheprimaseactivity,supplementreactionswithNTPs.

    

StorageConditions:

  

StorageTemperature:
-20ºC

Concentration:
1mg/ml

StorageBuffer:
20mMTrisHClpH7.5
100mMNaCl
0.1mMEDTA
1mMDTT
50%Glycerol
pH7.5@RT
      

QualityControl:

  


QualityAssuranceStatement:
T7Gp4AHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivityinfunctionalactivityassays.

            
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