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Biohelix/Thermostable Helicase/H0240S/20 µg

Description:

  


ThermostableDNAHelicaseisaDNAhelicasethatusestheenergygeneratedbythehydrolysisofnucleosidetriphosphatestobreakthehydrogenbondslinkingthetwostrandstogetherinduplexDNA.Itisactiveinatemperaturerangeof45to65ºC.

SUPPLIEDWITH:
10XAnnealingBufferII
100mMMgSO4

SuppliedbyUsers:ATP

      

Source:

  

PurifiedfromanE.colistrainthatoverexpressesthehelicasegeneisolatedfromathermophilicmicroorganism.      

Reactioncondition:

  


1XAnnealingBufferII:
10mMKCl
20mMTris-HCl,pH8.8@25°C

Supplementwith4mMMgSO4and3mMATP.
Incubateat45-65ºC.

    

StorageConditions:

  

StorageTemperature:
-20ºC

Concentration:
0.5mg/ml

StorageBuffer:
20mMTris-HClpH7.4
200mMNaCl
1mMDTT
0.1mMEDTA
50%Glycerol    

QualityControl:

  


QualityAssuranceStatement:
ThermostableHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivity.

            
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