Biohelix/IsoAmp® II Universal tHDA Kit/H0110S/50 reactions (50 µl reaction)

Description:

IsoAmpIIUniversaltHDAkitcanbeusedtoamplifyanddetectshortDNAsequences(70bp-120bp)ataconstanttemperature.Thekitcanbeusedwithavarietyoftemplates,includingmicrobialgenomicDNA,viralDNA,plasmidDNA,andCDNA.AsinglecopyoftargetDNAcanbeamplifiedbytHDAanddetectedbyagarosegelelectrophoresiswhenoptimizedprimersandbufferareused.

IsoAmpIIUniversaltHDAkitisbasedonasecond-generationThermophilicHelicase-DependentAmplificationplatform.ThereactionssupportedbyIsoAmp®IIUniversaltHDAkitincludetHDA,reversetranscriptionHDA(RT-HDA),real-timequantitativeHDA(qHDA)andreal-timequantitativeRT-HDA(qRT-HDA),fromasinglereactionbuffer.

IsoAmpIIUniversaltHDAkitcontainsanenzymemix,dNTPsolutionandbuffer,whichallowsflexibilityinreactionsetup.AControlTemplateandasetofspecificprimersaresuppliedforthepositivecontrolreaction.

Figure2.PositivecontrolassayswithvaryingamountofControlTemplate.
Positivecontrolassayswereconductedfollowingtheone-stepqHDAprotocol.ControlTemplatewasusedat5ng-500fgperassayintriplicate.

*Negativecontrolreactionsmayproduceprimer-dimer,whichcanbedistingushedfromspecificampliconsbygel-analysisorbymelting-curveanalysis.

Applications:

UsedtoamplifyanddetectshortDNAsequences(80-120bp)

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Advantages:
UseofhelicasetoseparateDNAeliminatesneedforthermocycler
Reactionscanbeperformedatconstanttemperature.
Workswithavarietyoftemplates,includingbacterialgenomicDNA,viralDNA,plasmidDNAandcDNA.
AslowasasinglecopyoftargetDNAcanbeamplifiedunderoptimizedconditions.
Canbeusedforreal-timequantitativeor/andreversetranscriptionHDA.

KitComponents:

IsoAmp®EnzymeMix(50reactions)
IsoAmp®dNTPSolution(50reactions)
10XAnnealingBufferII
MgSO₄(100mM)
NaCl(500mM)
pCNG1(ControlTemplate:1ng/µl)
NGF3(ForwardPrimer:5µM)
NGR3(ReversePrimer:5µM)

Note:ControlTemplateandPrimershavebeenchanged.

StorageConditions:

QualityControl:

QualityAssuranceStatement:
KitcomponentswereindividuallytestedforoptimalperformanceintHDA.

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