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Minerva-biolabs/Venor®GeM Classic/11-1250/250 tests

Venor®GeMClassicallowsfast,reliableandtime-savingroutinemonitoringofmycoplasmacontaminationbyPCR.

TypeofPCR

Forconventional,endpointPCR

RecommendedUse/Scope

ApplicableinresearchandindustryfordirecttestingofcellculturesandBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnostics.

KitComponents

Lyophilizedprimersetsandnucleotides
10xreactionbufferoptimizedforMBTaqDNAPolymerase
LyophilizedpositivecontrolDNA
Lyophilizedinternalamplificationcontrol

PackageSizes

Primersetsandnucleotidesarepreparedinaliquotsof25tests.

  • Cat.No.11-102525Tests
    Cat.No.11-105050Tests
    Cat.No.11-1100100Tests
    Cat.No.11-1250250Tests
Resultevaluation

GelelectrophoresisatendpointofPCR

RequiredConsumables

Polymerase.Wehighlyrecommendourreliablehot-startMBTaqDNAPolymerase,Cat.-No.53-0050/100/200/250.
PCRreactiontubes

OptionalforprocessvalidationandEP2.6.7complianttesting:
InternalControlDNAextra(4vialsfor300µleachofinternalamplificationcontrol;Cat.No.11-1905)
10CFU™SensitivityStandardsavailableforallEPlistedmycoplasmaspecies

Requiredlabdevices

RegularPCRcycler
AgarosegelelectrophoresisandDNAstainingsystem
Pipettingequipment
Tubecentrifuge

ShelfLifeandStorage

Componentsaremaintainableat+2to+8°Cforatleast6months.Afterrehydratisationthereagentsmustbestoredat-18°C.

EP2.6.7compliance

UseofVenor®GeMClassicforQAtestingofbiologicalslikemasterandworkingcellbanks,autologouscells,culturemedia,bulkharvestandfinalproducttestingaccordingtoEP2.6.7isapplicableafterappropriatesamplepreparationandprocessvalidation.

Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.

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