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Gibco/DMEM/F-12, powder/50 L/12500096

Description

Dulbecco"sModifiedEagleMedium:NutrientMixtureF-12(DMEM/F-12)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEM/F-12includeMDCK,glialcells,fibroblasts,humanendothelialcells,andratfibroblasts.WeofferavarietyofGibco®DMEM/F-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.

ThisDMEM/F-12ismodifiedasfollows:

WithWithout
•L-glutamine•HEPES
•PhenolRed•SodiumBicarbonate

Thecompleteformulationisavailable.

Gibco®DMEM/F-12isa1:1mixtureofDMEMandHam"sF-12.ThisformulationcombinesDMEM"shighconcentrationsofglucose,aminoacidsandvitaminswithF-12"swidevarietyofcomponents.

ProductIntendedUse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.

cGMPManufacturingandQualitySystem
Gibco®DMEM/F-12ismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferacomparableGibco®DMEM/F-12productmadeinourScotlandfacility(32500-035).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.

DMEM/F-12containsnoproteins,lipids,orgrowthfactors.Therefore,DMEM/F-12mayrequiresupplementation,commonlywith10%FetalBovineSerum(FBS).DMEM/F-12usesasodiumbicarbonatebuffersystemandthereforerequiresa5-10%CO2environmenttomaintainphysiologicalpH.

PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
ForInVitroDiagnosticUse.

Specifications

Glutamine:L-Glutamine
PhenolRedIndicator:PhenolRed
Form:Powder
HEPESBuffer:NoHEPES
SodiumPyruvateAdditive:SodiumPyruvate
SerumSupplementation:StandardSerumSupplementation
Glucose:HighGlucose
ProductLine:Gibco®
ProductSize:10x1L
ShippingCondition:RoomTemperature

Contents&storage

Storageconditions:2-8°C
Shippingconditions:Ambient
Shelflife:36monthsfromdateofmanufacture

Mediaformulations

12500-DMEM/F-12,powder
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