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Chromatrap/Chromatrap Premium Enzymatic ChIP qPCR Protein A/500167/1 Ea

KitContents:Chromatrap®ProteinAspincolumns(24),ColumnConditioningBuffer,WashBuffer1,WashBuffer2,WashBuffer3,1.3MGlycine,LysisBuffer,DigestionBuffer,EnzymaticStopSolution,Elutionsolution,0.1MNaOH,5MNaCl,1MNaHCO3,Hypotonicbuffer,ShearingCocktail,ProteaseInhibitorCocktail(PIC),ProteinaseKstopsolution,ProteinaseK,1.5mlCollectionTubes.ThepremiumkitalsoincludesPositiveAntibodyControl:HistoneH3,NegativeAntibodyControl:IgG,ForwardandReverseprimersetforGAPDH(humanpromoterregion).

Chromatrap®EnzymaticChIPkitsforqPCRrapidlyimproveChIPusingtheiruniquepatentedtechnologystreamliningtheprocess,ensuringchromatinpreparationtoqPCRanalysiscanbeperformedinunder5hours.

Byavoidingtheuseofmagneticoragarosebeads,non-specificbackgroundiseliminatedtogetherwithreducedmanualhandlingerrorsandsubsequentsampleloss.OptimisedelutionbufferchemistryallowssamplestobeanalyseddirectlyinqPCRwithouttheneedforDNAcleanup,furtherreducingsampleloss.

TheEnzymaticChIPkitisaperfectalternativeforthosewhocannotgetaccesstoasonicatortosheartheDNArequiredforaChIPassay.TheChIPqPCRassayisashortconvenientassayforlowchromatinloADIngwithverysmallslurryvolumes(40µls)withnoDNAcleanupstepspriortoqPCR.ThiskitisdesignedforuseasqPCRasthedownstreamprocessonlyandhasawidedynamicrangesuitableforlowchromatinloadings(1µg-10µg)

Optimisedfor1000ngsamplesizesallowingforbetterresultsfromsmallsamplesizesandprovidesgreaterflexibilitythusmoreIPassayscanbeperformedfromjustonesample
ThekitcontainssufficientProtein-AorProtein-Gbasedspin-columns,buffersandreagentstoperform24chromatinimmunoprecipitationassaysandreagentsforenzymaticshearingofchromatin(upto10preparations)
Ahighthroughputformatisalsoavailableallowingformultiplesampletype,antibodyandgenetargetstobetestedinparallel

Premiumkitscontainingpositiveandnegativeantibodyandprimersetsareavailabletoprovideyouwithsatisfactionofchromatinpreparation.

NotsureifourEnzymaticChIP-qPCRkitistherightchoiceforyourresearch?FindoutwhichChromatrap®ChIPkitisbestsuitedforyoubelow...

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Chromatrap/Chromatrap Premium Enzymatic ChIP qPCR Protein A/500167/1 Ea