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Chromatrap/Chromatrap Premium ChIP qPCR Protein A/500115/1 Ea

KitContents:Chromatrap®ProteinAspincolumns(24),ColumnConditioningBuffer,WashBuffer1,WashBuffer2,WashBuffer3,1.3MGlycine,LysisBuffer,HypotonicBuffer,Elutionsolution,0.1MNaOH,5MNaCl,1MNaHCO3,ProteaseInhibitorCocktail(PIC),ProteinaseKstopsolution,ProteinaseKand1.5mlCollectiontubes.ThepremiumkitalsoincludesPositiveAntibodyControl:HistoneH3,NegativeAntibodyControl:IgG,ForwardandReverseprimersetforGAPDH(humanpromoterregion).

Chromatrap®ChIPkitsforqPCRrapidlyimproveChIPusingtheiruniquepatentedtechnologystreamliningtheprocess,ensuringchromatinpreparationtoqPCRanalysiscanbeperformedinunder5hours.TheChIPqPCRassayisashortconvenientassayforlowchromatinloADIngwithverysmallslurryvolumes(40μls)withnoDNAcleanupstepspriortoqPCR.

Byavoidingtheuseofmagneticoragarosebeads,non-specificbackgroundiseliminatedtogetherwithreducedmanualhandlingerrorsandsubsequentsampleloss.OptimisedelutionbufferchemistryallowssamplestobeanalyseddirectlyinqPCRwithouttheneedforDNAcleanup,furtherreducingsampleloss.

Thekitshavebeenoptimisedfor1000ngsamplesizes,whichallowsforbetterresultsfromsmallersamplesizesandgreaterflexibilityasmoreIPassayscanbeperformedfromonesample.

  • DesignedforqPCRasadownstreamprocessonly
  • Suitableforlowchromatinloadings(1µg-10µg)
  • ContainssufficientProteinAorProteinGbasedspin-columns,buffersandreagentstoperform24chromatinimmunoprecipitations
  • Upto10chromatinpreparationsbysonication
  • Anenzymaticshearingformatisalsoavailable

Premiumkitscontainingpositiveandnegativeantibodyandprimersetstoprovideyouwithsatisfactionofchromatinpreparationareavailable.Ahighthroughputformatisalsoavailableallowingformultiplesampletype,antibodyandgenetargetstobetestedinparallel.

NotsureifourChIP-qPCRkitistherightchoiceforyourresearch?FindoutwhichChromatrap®ChIPkitisbestsuitedforyoubelow...

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