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Mediomics/10X KRB-IBMX Buffer/122937

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10XKRB-IBMXBuffersolutionisamixtureofa10XconcentrateofKrebs-RingerBicarbonateBuffer(KRB)supplementedwitha10Xconcentration(i.e.,7.5mM)of3-Isobutyl-1-Methylxanthine(IBMX)andcontaining10%ethanol.A1:10dilutionofthe10XKRB-IBMXBuffersolution(preparedasdescribedbelow)isrecommendedforuseinthepreparationofcells(eithersUSPensionoradherentcells)intendedforcAMPanalysisusingeitherMediomics’Bridge-ItTMcAMPdesignerfluorescentassayorBridge-ItTMcAMPallinonefluorescentassay.Whilethe1XbalancedsaltsolutionKRBservestoprovidecellswithwaterandessentialinorganicionstomaintainpHandosmoticbalance,theIBMXisapotentphosphodiesteraseinhibitorthatwillblockthedegradationofcAMP.

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