Immunology Consultants/anti-Bovine SAA Antibody (Mouse - Clone#1F12) - Monoclonal/1.0 mg/MSAA-10A-1F12

ProductDescriptionSize:100μlAbType:MonoclonalReactivity:AllImmunogen:NitratedKLHHost:MOUSE

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ProductDescriptionThemouse-derivedanti-3NTantibodyispurifiedwithProteinG.Itspecificallyrecognizesproteinswithtyrosinenitratedresiduesbutnotthetyrosinephosphorylatedornon-modifiedtyrosineresidues.BesidescommonapplicationsinWesternblottingandimmunoprecipitation,theantibodyhasbeenwellutilizedforproteomicscreeningtotyrosinenitratedsubstratesfromprotease-digestedproteinsbothinprokaryoticandeukaryoticcells.Source/PurificationThisproductisproducedbyimmunizingmicewithnitratedKLH.TheasciteswaspurifiedbyproteinG.SpecificityAllnitratedproteinsexpectedRecommendedApplicationsELISA,WB,IP*WB=Westernblotting; IP=ImmunoprecipitationRecommendedantibodydilutionWB:1:1000ImportantForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Useatanassaydependentconcentration.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.Notyettestedinotherapplications.Dotblotting analysisonindicatedamountofpeptidesusing3-nitrotyrosinemousemAb.Thelistofpeptidesisincludedinthetableontheright.Westernblot analysison30μgofextractsfromHeLaandMEFwholecelllysates,untreatedortreatedwithperoxynitrite(3mM,30mins)orpervanadate(3mM,30mins),usinganti-3-nitrotyrosinemouseAntibody(FigureB)oranti-phospho-TyrosinerabbitpAb(PTM-702)(FigureC).ScientificDescriptionNitrotyrosineisaproductoftyrosinenitrationmediatedbyreactivenitrogenspeciessuchasperoxynitriteanionandnitrogendioxide.ItisverystableandsoissuitableforanalysisinBIOLOGicalsamplesuchasplasma,lungaspirants-BALF(Bronchoalveolarliningfluid)andurine.IncreasednitrotyrosinelevelshavebeenassociatedwithseveralphysiologicalandpathologicalconditionsandisconsideredaMarkerofNO-dependent,reactivenitrogenspecies-inducednitrativestress.ResearchshowsthatnitrotyrosinelevelscanbereducedbyN-acetylcysteinewhichisaprecursortoglutathione,oneofthebody’sprimaryendogenousantioxidants.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals.