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BioVision/Glycine Oxidase H244K, Bacillus subtilis recombinant/7845/

Alternate Name Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
Gene Symbol thiO
Gene ID 939377
Accession # O31616
Source E. coli
Appearance Liquid
Physical Form Description 4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol
Molecular Weight 43.1 kDa (1-369 aa, NT His Tag)
Purity by SDS-PAGE ≥90%
Biological Activity 1200 mU/mg
Amino Acid Sequence 1-369 aa
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Description:

Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
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