HumanIgMELISpotBASICenablesenumerationofBcellssecretingtotalhumanIgMaswellasantigen-specifichumanIgM.Itcontainsmatchedquantitiesofmonoclonalcaptureanddetectionantibodies,Streptavidin-ALPandpolyclonalactivators.
Antigen-specificBcellscanbeenumeratedintwoalternativeways;bycoatingwithantigenorbyusingbiotinylatedantigeninthedetectionstep.FormemoryBcells,werecommendpre-stimulationwithR848andrecombinanthumanIL-2(bothincluded).
HumanIgMELISpotBASICisalsoavailablewithStreptavidin-HRP.SeeRelatedproducts.
Ascomplementaryproducts,weofferELISpotplatesandsubstrates.SeeRelatedproducts.
IgM
MonomericIgM(ImmunoglobulinM)isfoundonthesurfaceofBcellswhereastheIgMmoleculessecretedfromplasmaBcellsarepentamers,i.e.fiveIgMmoleculesjoinedtogetherbytheJchain.IgMconstitutesapproximately10%ofthetotalIgconcentrationinserum.IgMisthefirstantibodyisotypeinducedinatypicalimmuneresponseandhenceistheisotypewiththelowestaffinityforantigens.Duetoitsstructure,with10antigen-bindingsitesperIgMpentamer,itcanneverthelessbinde.g.surface-boundantigenswithaveryhighAvidity.
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| Otherformats: | ClicktoviewallHuman/NHPIgMproducts |
IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
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