Thiskitisidealforuserswhowantaconvenientandsensitiveassay.TheassayisdesignedfortheenumerationofcellssecretinghumanIFN-γ.ThekitincludesELISpotplatespre-coatedwithmonoclonalantibody,biotinylateddetectionantibody,Streptavidin-HRPandready-to-useTMBsubstrate.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.
Pre-coatedplatesreduceassaytimeandminimizevariABIlity.
HumanIFN-γELISpotPLUSkitsarealsoavailablewithStreptavidin-ALPandBCIP/NBT-plussubstrate.SeeRelatedproducts.
IFN-γ
Interferon-γ(IFN-γ,alsoknownasIFN-gamma,IFN-g,IFNgandInterferon-gamma)isproducedmainlybyactivatedTcellsandNKcells.Itisaproinflammatorycytokinethatactivatesmacrophagesandendothelialcells,butitalsoregulatesimmuneresponsesbyeffectingAPCandTandBcells.ProductionofIFN-γbyhelperTcellsaswellascytotoxicTcellsisahallmarkoftheTH1-typephenotype,thus,high-levelproductionofIFN-γistypicallyassociatedwitheffectivehostdefenseagainstintracellularpathogens.
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| Size: | 2plates |
| Otherformats: | ClicktoviewallHumanIFN-γproducts |
| Minimizeworkloadwiththeone-stepdetectionELISpotPROkit | |
WhatcanIsay...Mabtechproductsaregreat.Optimizedprotocols,reproducIBLeresults.WeusetheELISpotIFNgkits(human,primate,dog,rat)inroutineforourresearchprojectsbutalsoforourGeneTherapyImmunologyplatform.Julia,oursalescontact,isamazingandveryresponsive.ChristianhelpedusalotforourreaderusingandsowewearefinallylaunchingintotheFluoroSpotadventure!
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