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PEPperPRINT/PEPperMAP® Cross-Reactivity Analysis/PPM.006.003

MicroarrayContent:PEPperCHIP®HumanEpitomeMicroarraywithallhumanlinearB-cellepitopesoftheImmuneEpitopeDatabaseassociatedwithinfectiousdiseases(8,504epitopes),autoimmunediseases(3,808epitopes),allergicdiseases(3,451epitopes),transplantdiseases(64epitopes),healthycontrols(1,481epitopes),cancer(199epitopes),otherdiseases(995epitopes)andthemostcommonvaccines(4,661epitopes).

ThePEPperMAP®Cross-ReactivityAnalysisofamono-orpolyclonalantibodywiththePEPperCHIP®HumanEpitomeMicroarrayisbasedonthree-stepapproachandincludes:

  1. ImmunoassayswiththePEPperCHIP®HumanEpitomeMicroarrayfollowedbystainingwithasecondaryantibodyanddataread-out
  2. Bioinformaticidentificationofconsensusmotifs,essentialandconservedaminoacidpositionsamongthetoppeptidehitswiththeMEMEtool(MultipleEmforMotifElicitation)oftheMEMESuite
  3. FIMOanalysisoftheMEMEconsensusmotifwithaproteindatabaseblasttosearchforpossIBLeproteincross-reactionssortedbycross-reactionprobABIlities.

TheresultsofthePEPperMAP®Cross-ReactivityAnalysisaresummarizedinadetailedscientificreportincludingadatadiscussionandanevaluationofthecross-reactivityofamono-orpolyclonalantibody.

RelatedProducts:PEPperMAP®LinearEpitopeMapping,PEPperMAP®ConformationalEpitopeMapping,PEPperMAP®EpitopeSubstitutionScan

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