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HumanZyme/Recombinant Human NT-4 (Neurotrophin-4)/HZ-7273/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/Recombinant Human NT-4 (Neurotrophin-4)/HZ-7273/100 µg

ProductDescription:

NT-4isaneurotrophicfactorstructurallyrelatedtoβ-NGF,BDNF,andNT-3.Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.NT-4isexpressedintheprostate,thymus,placenta,andskeletalmuscle.NT-4cansignalthroughtheLNGFRandtrkBreceptors,andpromotesthesurvivalofperipheralsensorysympatheticneurons.RecombinantHumanNT-4isanoncovalentlylinkedhomodimeroftwo14.0kDapolypeptidemonomers(260totalaminoacidresidues).

TechnicalSpecifications:

  • Species:Human
  • Expression:E.ColiCellExpressed
  • Activity: Typically20-50ng/mlED50
  • Purity:≥98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:14kDa
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

TheED50asdeterminedbythedose-dependentinductionofcholineacetyltransferaseactivityinratbasalforebrainprimaryseptalcellcultureswasfoundintherangeof20-50ng/ml.

AASequence:

GVSETAPASRRGELAVCDAVSGWVTDRRTA
VDLRGREVEVLGEVPAAGGSPLRQYFFETR
CKADNAEEGGPGAGGGGCRGVDRRHWVSEC
KAKQSYVRALTADAQGRVGWRWIRIDTACV
CTLLSRTGRA

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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