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HumanZyme/IL-34 (Interleukin-34)/HZ-7064/500 µg

ProductDescription:

IL-34isahomodimericcytokinethatisexpressedinarangeoftissuesthatincludespleen,heart,brain,liver,kidney,lung,ovary,thymus,testis,smallintestine,prostateandcolon.IL-34isaligandforcolony-stimulatingfactor-1receptor(CSF1R),whichalsobindstoCSF-1.ItspecificallybindsCD14+monocytes,promotessurvivalandproliferationofhumanperipheralbloodmonocytes,andstimulatesmacrophagecolonyformationbyhumanbonemarrowcells.IL-34,likehumanCSF-1,stimulatesphosphorylationofMAPK1/ERK2andMAPK3/ERK1.RecombinantHumanIL-34isa52.5kDahomodimericglycoproteinconsistingof460aminoacidresidues,includingaC-terminalHis-Tag.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically≤0.5ng/mLED50
  • Purity:>95%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:52.5kDa,homodimeric,glycoprotein
  • Formulation:20mMSodiumCitrate,pH3.0+75mMNaCl
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

MeasuredbyitsABIlitytoinducesecretionofMCP-1fromPBMCs.

AASequence:

NEPLEMWPLTQNEECTVTGFLRDKLQYRSR
LQYMKHYFPINYKISVPYEGVFRIANVTRL
QRAQVSERELRYLWVLVSLSATESVQDVLL
EGHPSWKYLQEVETLLLNVQQGLTDVEVSP
QGLTDVEVSPAPGPNLKLVRPKALLDNCFR
VMELLYCSCCKQSSVLNWQDCEVPSPQSCS
PEPSLQYAATQLYPPPPWSPSSPPHSTGSV
RPVRAQGEGLLPHHHHHHHH

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Thissolutioncanbestoredat2-8°Cforupto1week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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