FGF-10isaheparin-bindinggrowthfactorthatbelongstotheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-10ismostrelatedtoKGF/FGF-7,andisexpressedduringthedevelopmentand,preferentially,inadultlungs.ItsignalsthroughtheFGFR2b.
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤0.5ng/ml,correspondingtoaspecificactivityof≥2x106units/mg.
MLGQDMVSPE | ATNSSSSSFS | SPSSAGRHVR |
SYNHLQGDVR | WRKLFSFTKY | FLKIEKNGKV |
SGTKKENCPY | SILEITSVEI | GVVAVKAINS |
NYYLAMNKKG | KLYGSKEFNN | DCKLKERIEE |
NGYNTYASFN | WQHNGRQMYV | ALNGKGAPRR |
GQKTRRKNTS | AHFLPMVVHS |
Centrifugethevialpriortoopening.5mMSodiumPhosphate,pH7.4toaconcentrationof0.1-1.0mg/ml.Donotvortex.
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g