CNTFisapotentneuralfactorthatwasoriginallycharacterizedasavitalfactorforthesurvivalofchickciliaryneuronsinvitro.CNTFisalsoimportantforthesurvivalofotherneuralcelltypes,includingprimarysensoryneurons,motorneurons,basalforebrainneuronsandtype2astrocytes.CNTFishighlyconservedacrossspeciesandexhibitscross-speciesbioactivity.RecombinantHumanCNTFissynthesizedasa199aminoacidpolypeptidelackingahydrophobicN-terminalsignalforsecretion.
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
DeterminedbyitsABIlitytostimulateproliferationofhumanTF-1cellsusinganED50concentrationrangeof50-150ng/ml.
AFTEHSPLTP | HRRDLCSRSI | WLARKIRSDL |
TALTQSYVKH | QGLNKNINLD | SADGMPVAST |
DQWSQLTQAQ | RLQQNLQAYR | TFHVLLARLL |
QDQQVHFTPT | QGDFHQAIHT | LLLQVAAFAY |
QIQQLMILLQ | YKIPRNQADG | MPINVGDGGL |
FQKKLWGLKV | LQQLSQWTVR | SIHDLRFISS |
HQTGIPARGS | HYIANNKKM |
Centrifugethevialpriortoopening.Reconstitutein5-10mMSodiumPhosphate,pH7.5toaconcentrationof0.1-1.0mg/ml.Donotvortex.
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g