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HumanZyme/CNTF (Ciliary Neurotrophic Factor)/HZ-7316/500 µg

 ProductDescription:

CNTFisapotentneuralfactorthatwasoriginallycharacterizedasavitalfactorforthesurvivalofchickciliaryneuronsinvitro.CNTFisalsoimportantforthesurvivalofotherneuralcelltypes,includingprimarysensoryneurons,motorneurons,basalforebrainneuronsandtype2astrocytes.CNTFishighlyconservedacrossspeciesandexhibitscross-speciesbioactivity.RecombinantHumanCNTFissynthesizedasa199aminoacidpolypeptidelackingahydrophobicN-terminalsignalforsecretion.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Activity:Typically50-150ng/mLED50
  • Purity:≥98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:22.8kDa
  • Formulation:5mMSodiumPhosphatepH7.5+75mMNaCl
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

DeterminedbyitsABIlitytostimulateproliferationofhumanTF-1cellsusinganED50concentrationrangeof50-150ng/ml.

AASequence:

AFTEHSPLTPHRRDLCSRSIWLARKIRSDL
TALTQSYVKHQGLNKNINLDSADGMPVAST
DQWSQLTQAQRLQQNLQAYRTFHVLLARLL
QDQQVHFTPTQGDFHQAIHTLLLQVAAFAY
QIQQLMILLQYKIPRNQADGMPINVGDGGL
FQKKLWGLKVLQQLSQWTVRSIHDLRFISS
HQTGIPARGSHYIANNKKM

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstitutein5-10mMSodiumPhosphate,pH7.5toaconcentrationof0.1-1.0mg/ml.Donotvortex.

Storage:

Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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