TGF-βfamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesis,andapoptosis.Asimpliedbytheirname,BMPsinitiate,promote,andregulatethedevelopment,growth,andremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodeling,andmaintenanceofavarietyofothertissuesandorgans.IncreasingevidenceindicatesthatBMP-Smadsignalinghasatumorsuppressingactivity,andthatBMPscaninhibittumorgrowth.BMP-6isabnormallyexpressedinbreastcancercelllines,however,itsfunctioninpromotingbreastcancerdevelopmentisunknown.ThematureandfunctionalformofBMP-6isahomodimeroftwoidentical139aminoacidpolypeptidechainslinkedbyasingledisulfidebond.EachmonomerisexpressedastheC-terminalpartofaprecursorpolypeptide,whichcontainsa20aminoacidsignalpeptideanda354aminoacidpropeptide.Thisprecursorundergoesintracellulardimerization,anduponsecretionitisprocessedbyafurin-typeprotease.
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5cells.
VSSASDYNSS | ELKTACRKHE | LYVSFQDLGW |
QDWIIAPKGY | AANYCDGECS | FPLNAHMNAT |
NHAIVQTLVH | LMNPEYVPKP | CCAPTKLNAI |
SVLYFDDNSN | VILKKYRNMV | VRACGCH |
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g